desulfitobacterium_pce_ii_medium

CultureMech:000495 bacterial COMPLEX LIQUID
Original name: DESULFITOBACTERIUM (PCE II) MEDIUM

Overview

External ontology
mediadive.medium:1062 (source link unavailable) — DESULFITOBACTERIUM (PCE II) MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1062.pdf

Composition

flowchart LR
    CultureMech_000495["desulfitobacterium_pce_ii_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000495 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000495 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_NaCl["NaCl"]
    CultureMech_000495 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000495 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_KCl["KCl"]
    CultureMech_000495 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000495 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000495 --> ing6_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Sodium_resazurin -.-> CHEBI_8806
    ing7_Na2CO3["Na2CO3"]
    CultureMech_000495 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000495 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_000495 --> ing9_Yeast_extract
    ing10_Na_pyruvate["Na-pyruvate"]
    CultureMech_000495 --> ing10_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing10_Na_pyruvate -.-> CHEBI_50144
    ing11_Na2_fumarate["Na2-fumarate"]
    CultureMech_000495 --> ing11_Na2_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing11_Na2_fumarate -.-> CHEBI_115156
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000495 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000495 --> ing13_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing14_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000495 --> ing14_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing14_MnSO4_x_H2O -.-> CHEBI_86364
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000495 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000495 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000495 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000495 --> ing18_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing19_H3BO3["H3BO3"]
    CultureMech_000495 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000495 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000495 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000495 --> ing22_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000495 --> ing23_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000495 --> ing24_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing25_D_____biotin["D-(+)-biotin"]
    CultureMech_000495 --> ing25_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing25_D_____biotin -.-> CHEBI_15956
    ing26_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000495 --> ing26_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.197824 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.24728 G_PER_L CHEBI:31206
ammonium chloride
3 NaCl
Cl.Na 58.443 g/mol
1.98912 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 0.98912, 1.0]
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.395648 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 KCl
Cl.K 74.551 g/mol
0.49456 G_PER_L CHEBI:32588
potassium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.248368 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.148368, 0.1]
7 Sodium resazurin
C12H7NO4 229.191 g/mol
0.00049456 G_PER_L CHEBI:8806
Resazurin
8 Na2CO3
CO3.2Na 105.988 g/mol
1.48368 G_PER_L CHEBI:29377
sodium carbonate
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.12176060000000001 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.0217606, 0.1]
10 Yeast extract 1.97824 G_PER_L
11 Na-pyruvate
C3H3O3.Na 110.044 g/mol
4.45104 G_PER_L CHEBI:50144
sodium pyruvate
12 Na2-fumarate
C4H2O4.2Na 160.036 g/mol
6.42928 G_PER_L CHEBI:115156
disodium fumarate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
15 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
23 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
24 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
25 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
26 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
27 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Dissolve ingredients (except carbonate, ferrous iron sulfate, vitamins and organic substrates), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add ferrous iron sulfate (dissolved in 0.1 N H2SO4), vitamins, pyruvate, fumarate and yeast extract from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Stock solutions of ferrous iron sulfate, vitamins, pyruvate and fumarate should be sterilized by filtration. Adjust pH of the complete medium to 7.5, if necessary.
  2. Note: Addition of 10 - 20 mg sodium dithionite per litre (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Solutions

Seven vitamins solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.1 G_PER_L
Nicotinic acid 0.2 G_PER_L
Pyridoxine hydrochloride 0.3 G_PER_L
Thiamine-HCl x 2 H2O 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.270984Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1062
2026-03-13T03:08:58.133113Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:18:55.840967Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000495
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.858558+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFITOBACTERIUM (PCE II) MEDIUM" to "desulfitobacterium_pce_ii_medium" for consistent matching
2026-06-05T07:40:56.015562+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.344611+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:21.884289+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Seven vitamins solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).