brevibacillus_levickii_medium (CultureMech:000497)

Overview

External ontology

mediadive.medium:1064 (source link unavailable) — BREVIBACILLUS LEVICKII MEDIUM

Applications

Microbial cultivation

pH

5.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1064.pdf

Composition

flowchart LR
    CultureMech_000497["brevibacillus_levickii_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000497 --> ing0_Yeast_extract
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000497 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000497 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000497 --> ing3_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing3_MnSO4_x_H2O -.-> CHEBI_86364
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000497 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000497 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Agar["Agar"]
    CultureMech_000497 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

#	Ingredient	Concentration	Ontology
1	Yeast extract	2 G_PER_L
2	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.25 G_PER_L	CHEBI:62946 ammonium sulfate
3	KH2PO4 H2O4P.K 136.084 g/mol	1.5 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
4	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.0025 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.1 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
6	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.0125 G_PER_L	CHEBI:86158 calcium chloride dihydrate
7	Agar	18 G_PER_L	CHEBI:2509 agar

Preparation

Adjust pH to 5.5; autoclave at 121° for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:13.279577Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1064
2026-03-13T06:18:55.860587Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000497
2026-03-17T06:30:46.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.880424+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "BREVIBACILLUS LEVICKII MEDIUM" to "brevibacillus_levickii_medium" for consistent matching
2026-06-05T07:40:51.541957+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.970948+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.