aml60_medium

CultureMech:000505 bacterial DEFINED LIQUID
Original name: AML60 MEDIUM

Overview

External ontology
mediadive.medium:1073 (source link unavailable) — AML60 MEDIUM
Applications
  • Microbial cultivation
pH
9.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1073.pdf

Composition

flowchart LR
    CultureMech_000505["aml60_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000505 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgSO4["MgSO4"]
    CultureMech_000505 --> ing1_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing1_MgSO4 -.-> CHEBI_32599
    ing2_NaCl["NaCl"]
    CultureMech_000505 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000505 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_KH2PO4["KH2PO4"]
    CultureMech_000505 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Na2CO3["Na2CO3"]
    CultureMech_000505 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_NaHCO3["NaHCO3"]
    CultureMech_000505 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_HCl["HCl"]
    CultureMech_000505 --> ing7_HCl
    CHEBI_17883["CHEBI:17883"]
    ing7_HCl -.-> CHEBI_17883
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000505 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_ZnCl2["ZnCl2"]
    CultureMech_000505 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000505 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_000505 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000505 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000505 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000505 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000505 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_NaOH["NaOH"]
    CultureMech_000505 --> ing16_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing16_NaOH -.-> CHEBI_32145
    ing17_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000505 --> ing17_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing17_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000505 --> ing18_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.19802 G_PER_L CHEBI:62946
ammonium sulfate
2 MgSO4
Mg.O4S 120.368 g/mol
0.049505 G_PER_L CHEBI:32599
magnesium sulfate
3 NaCl
Cl.Na 58.443 g/mol
118.812 G_PER_L CHEBI:26710
sodium chloride
4 K2HPO4
HO4P.2K 174.174 g/mol
0.29703 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 KH2PO4
H2O4P.K 136.084 g/mol
0.158416 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 Na2CO3
CO3.2Na 105.988 g/mol
21.2 G_PER_L CHEBI:29377
sodium carbonate
7 NaHCO3
CHO3.Na 84.006 g/mol
8.4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
9 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
10 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
17 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
18 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
19 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients (except carbonates, vitamins, arsenate and sulfide), boil medium for 1 min., then cool to room temperature under N2 gas atmosphere. Add solid carbonates and adjust pH to 9.8, then dispense under same gas atmosphere in culture vessels and autoclave.
  2. Prior to inoculation, add vitamins, arsenate and sulfide from sterile, anaerobic stock solutions prepared under N2 and check pH of the completed medium. The vitamin solution should be sterilized by filtration.
  3. Growth of strain DSM 17814 takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy.
  4. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.295941Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1073
2026-03-13T06:18:55.913779Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000505
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.937751+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AML60 MEDIUM" to "aml60_medium" for consistent matching
2026-06-05T07:40:48.532815+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.044378+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.