Overview
- External ontology
- mediadive.medium:1076 (source link unavailable) — SP4 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1076.pdf
Composition
flowchart LR
CultureMech_000508["sp4_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_000508 --> ing0_Tryptone
ing1_Peptone["Peptone"]
CultureMech_000508 --> ing1_Peptone
ing2_PPLO_broth["PPLO broth"]
CultureMech_000508 --> ing2_PPLO_broth
ing3_Fetal_bovine_serum["Fetal bovine serum"]
CultureMech_000508 --> ing3_Fetal_bovine_serum
ing4_CMRL_1066["CMRL 1066"]
CultureMech_000508 --> ing4_CMRL_1066
ing5_Yeast_extract["Yeast extract"]
CultureMech_000508 --> ing5_Yeast_extract
ing6_Phenol_red["Phenol red"]
CultureMech_000508 --> ing6_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing6_Phenol_red -.-> CHEBI_31991
ing7_L_Glutamine["L-Glutamine"]
CultureMech_000508 --> ing7_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing7_L_Glutamine -.-> CHEBI_18050
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 9.70874 G_PER_L |
MICRO:0000182 (source link unavailable)
Tryptone
|
|
| 2 | Peptone | 5.14563 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
|
| 3 | PPLO broth | 3.39806 G_PER_L | ||
| 4 | Fetal bovine serum | 90 G_PER_L | ||
| 5 | CMRL 1066 | 25 G_PER_L | ||
| 6 | Yeast extract | 4.5 G_PER_L |
FOODON:03315426
Yeast extract
|
[Merged 2 duplicates: 3.5, 1.0] |
| 7 |
Phenol red
C19H14O5S 354.383 g/mol
|
0.01 G_PER_L |
CHEBI:31991
phenol red
|
|
| 8 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
0.0496876 G_PER_L |
CHEBI:18050
L-glutamine
|
Preparation
- Adjust pH to 7.4; autoclave at 121°C for 15 minutes.
- Add aseptically:
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.305654Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1076 |
| 2026-03-13T03:09:39.155795Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:55.941578Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000508 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.967698+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SP4 MEDIUM" to "sp4_medium" for consistent matching |
| 2026-06-05T07:41:46.118828+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch7.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH7 | Applied local mappings for Peptone, Tryptone, Yeast extract. |