friis_medium (CultureMech:000512)

Overview

External ontology

mediadive.medium:1078 (source link unavailable) — FRIIS MEDIUM

Applications

Microbial cultivation

pH

7.4

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1078.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_000512["friis_medium"]
    ing0_Calf_brains["Calf brains"]
    CultureMech_000512 --> ing0_Calf_brains
    ing1_Beef_heart["Beef heart"]
    CultureMech_000512 --> ing1_Beef_heart
    ing2_Proteose_peptone["Proteose peptone"]
    CultureMech_000512 --> ing2_Proteose_peptone
    ing3_Dextrose["Dextrose"]
    CultureMech_000512 --> ing3_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing3_Dextrose -.-> CHEBI_17634
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_000512 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710
    ing5_Disodium_phosphate["Disodium phosphate"]
    CultureMech_000512 --> ing5_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing5_Disodium_phosphate -.-> CHEBI_34683
    ing6_PPLO_broth["PPLO broth"]
    CultureMech_000512 --> ing6_PPLO_broth
    ing7_Porcine_serum["Porcine serum"]
    CultureMech_000512 --> ing7_Porcine_serum
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_000512 --> ing8_Yeast_extract
    ing9_Phenol_red["Phenol red"]
    CultureMech_000512 --> ing9_Phenol_red
    CHEBI_31991["CHEBI:31991"]
    ing9_Phenol_red -.-> CHEBI_31991
    ing10_NaCl["NaCl"]
    CultureMech_000512 --> ing10_NaCl
    ing10_NaCl -.-> CHEBI_26710
    ing11_KCl["KCl"]
    CultureMech_000512 --> ing11_KCl
    CHEBI_32588["CHEBI:32588"]
    ing11_KCl -.-> CHEBI_32588
    ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000512 --> ing12_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing13_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000512 --> ing13_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing13_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing14_CaCl2["CaCl2"]
    CultureMech_000512 --> ing14_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing14_CaCl2 -.-> CHEBI_3312
    ing15_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_000512 --> ing15_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing15_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing16_KH2PO4["KH2PO4"]
    CultureMech_000512 --> ing16_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing16_KH2PO4 -.-> CHEBI_63036

Ingredients (17)

#	Ingredient	Concentration	Ontology	Notes
1	Calf brains Difco/BD Brain Heart Infusion 237500	variable VARIABLE	UBERON:0000955 brain	infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product
2	Beef heart Difco/BD Brain Heart Infusion 237500	variable VARIABLE	UBERON:0000948 heart	infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product
3	Proteose peptone Difco/BD Brain Heart Infusion 237500	10.0 G_PER_L	MICRO:0000180 (source link unavailable) proteose peptone	Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product
4	Dextrose C6H12O6 180.156 g/mol Difco/BD Brain Heart Infusion 237500	2.0 G_PER_L	CHEBI:17634 D-glucose	Carbon/energy source; Constituent of Brain Heart Infusion commercial product
5	Sodium chloride Cl.Na 58.443 g/mol Difco/BD Brain Heart Infusion 237500	5.0 G_PER_L	CHEBI:26710 sodium chloride	Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product
6	Disodium phosphate HO4P.2Na 141.958 g/mol Difco/BD Brain Heart Infusion 237500	2.5 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product
7	PPLO broth	5.40373 G_PER_L
8	Porcine serum	30 G_PER_L
9	Yeast extract	0.83 G_PER_L
10	Phenol red C19H14O5S 354.383 g/mol	0.00133 G_PER_L	CHEBI:31991 phenol red
11	NaCl Cl.Na 58.443 g/mol	160 G_PER_L	CHEBI:26710 sodium chloride
12	KCl Cl.K 74.551 g/mol	8 G_PER_L	CHEBI:32588 potassium chloride
13	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	2 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
14	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	2 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
15	CaCl2 Ca.2Cl 110.984 g/mol	3.7 G_PER_L	CHEBI:3312 calcium dichloride
16	Na2HPO4 x 2 H2O 2H2O.HO4P.2Na 177.988 g/mol	3 G_PER_L	CHEBI:91258 disodium hydrogenphosphate dihydrate
17	KH2PO4 H2O4P.K 136.084 g/mol	1.2 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate

Preparation

Adjust to pH 7.4.
After autoclaving add:
25 ml solution 1 (4 g NaCl, 0.2 g KCl, 0.05 g MgSO4 x 7 H2O, 0.05 g MgCl2 x 6 H2O dissolved in 25 ml distilled water; then 0.0925 g CaCl2 added and dissolved) and 25 ml of solution 2 (0.075 g Na2HPO4 x 2 H2O, 0.03 g KH2PO4 dissolved in 25 ml distilled water) are autoclaved separately and then combined with 450 ml sterile H2O.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:13.312522Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1078
2026-03-13T06:18:55.963361Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000512
2026-03-17T06:30:40.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.987798+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "FRIIS MEDIUM" to "friis_medium" for consistent matching
2026-06-05T07:41:05.544761+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:58.826996+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.