Overview
- External ontology
- mediadive.medium:1079 (source link unavailable) — MYCOPLASMA/ACHOLEPLASMA MEDIUM (CIP MEDIUM 88)
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.4, 'max': 7.8}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1079.pdf
Composition
flowchart LR
CultureMech_000513["mycoplasma_acholeplasma_medium_cip_medium_88"]
ing0_PPLO_broth["PPLO broth"]
CultureMech_000513 --> ing0_PPLO_broth
ing1_Yeast_extract["Yeast extract"]
CultureMech_000513 --> ing1_Yeast_extract
ing2_Phenol_red["Phenol red"]
CultureMech_000513 --> ing2_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing2_Phenol_red -.-> CHEBI_31991
ing3_Glucose["Glucose"]
CultureMech_000513 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Horse_serum["Horse serum"]
CultureMech_000513 --> ing4_Horse_serum
ing5_Ampicillin["Ampicillin"]
CultureMech_000513 --> ing5_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing5_Ampicillin -.-> CHEBI_28971
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | PPLO broth | 17 G_PER_L | ||
| 2 | Yeast extract | 25 G_PER_L | ||
| 3 |
Phenol red
C19H14O5S 354.383 g/mol
|
0.2 G_PER_L |
CHEBI:31991
phenol red
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Horse serum | 200 G_PER_L | ||
| 6 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
4.7619 G_PER_L |
CHEBI:28971
ampicillin
|
Preparation
- Add all components except sterile supplement; adjust pH to 7.6 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement.
- Adjust pH to 7.0 + 0.2 and autoclave at 121°C for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.314852Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1079 |
| 2026-03-13T06:18:55.967913Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000513 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.992801+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MYCOPLASMA/ACHOLEPLASMA MEDIUM (CIP MEDIUM 88)" to "mycoplasma_acholeplasma_medium_cip_medium_88" for consistent matching |
| 2026-05-20T06:02:00.560611+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.4-7.8' -> {'min': 7.4, 'max': 7.8} |
| 2026-06-05T07:41:39.027237+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |