Overview
- External ontology
- mediadive.medium:1085 (source link unavailable) — HOWARDELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1085.pdf
Composition
flowchart LR
CultureMech_000519["howardella_medium"]
ing0_Casitone["Casitone"]
CultureMech_000519 --> ing0_Casitone
ing1_Yeast_extract["Yeast extract"]
CultureMech_000519 --> ing1_Yeast_extract
ing2_Na2HPO4["Na2HPO4"]
CultureMech_000519 --> ing2_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing2_Na2HPO4 -.-> CHEBI_34683
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000519 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_000519 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_Urea["Urea"]
CultureMech_000519 --> ing5_Urea
CHEBI_16199["CHEBI:16199"]
ing5_Urea -.-> CHEBI_16199
ing6_Na_thioglycolate["Na-thioglycolate"]
CultureMech_000519 --> ing6_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing6_Na_thioglycolate -.-> CHEBI_86481
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casitone | 20 G_PER_L | ||
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Urea
CH4N2O 60.056 g/mol
|
1 G_PER_L |
CHEBI:16199
urea
|
|
| 7 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.75 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Preparation
- Dissolve ingredients (except urea and thioglycolate), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add thioglycolate, dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add urea from a 10% (w/v) stock solution sterilized by filtration and stored under 100% N2 gas. Adjust pH of complete medium to 7.4.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.331239Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1085 |
| 2026-03-13T06:18:56.008145Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000519 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.037884+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HOWARDELLA MEDIUM" to "howardella_medium" for consistent matching |
| 2026-06-05T07:41:08.722876+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.820370+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |