Overview
- External ontology
- mediadive.medium:108 (source link unavailable) — ACIDIPHILIUM ACIDOPHILUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 4.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium108.pdf
Composition
flowchart LR
CultureMech_000521["acidiphilium_acidophilum_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_000521 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_000521 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000521 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_000521 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_000521 --> ing4_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000521 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Agar["Agar"]
CultureMech_000521 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_D_Glucose["D-Glucose"]
CultureMech_000521 --> ing7_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing7_D_Glucose -.-> CHEBI_17634
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.018 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1e-05 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for solid medium) |
| 8 |
D-Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17634
D-glucose
|
Preparation
- In liquid medium, the pH should be adjusted to 3.5 with H2SO4. In solid medium, the pH should be adjusted to 4.5 with H2SO4 after autoclaving the medium. Sterilize the glucose separately and add to the sterile medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.181509Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 108 |
| 2026-03-13T06:18:56.029628Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000521 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.061294+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDIPHILIUM ACIDOPHILUM MEDIUM" to "acidiphilium_acidophilum_medium" for consistent matching |
| 2026-06-05T07:40:47.303015+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.597072+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |