archaeoglobus_infectus_medium

Overview

External ontology

mediadive.medium:1098 (source link unavailable) — ARCHAEOGLOBUS INFECTUS MEDIUM

Applications

• Microbial cultivation

pH

6.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1098.pdf

Composition

flowchart LR
    CultureMech_000529["archaeoglobus_infectus_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_000529 --> ing0_Sea_Salt
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000529 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000529 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Na_acetate["Na-acetate"]
    CultureMech_000529 --> ing3_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Na_acetate -.-> CHEBI_32954
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_000529 --> ing4_Yeast_extract
    ing5_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000529 --> ing5_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Sodium_resazurin -.-> CHEBI_8806
    ing6_NaHCO3["NaHCO3"]
    CultureMech_000529 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
    CultureMech_000529 --> ing7_2_Mercaptoethanesulfonate
    CHEBI_17905["CHEBI:17905"]
    ing7_2_Mercaptoethanesulfonate -.-> CHEBI_17905
    ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000529 --> ing8_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing9_DL_Dithiothreitol["DL-Dithiothreitol"]
    CultureMech_000529 --> ing9_DL_Dithiothreitol
    CHEBI_18320["CHEBI:18320"]
    ing9_DL_Dithiothreitol -.-> CHEBI_18320
    ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000529 --> ing10_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing11_NaCl["NaCl"]
    CultureMech_000529 --> ing11_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing11_NaCl -.-> CHEBI_26710
    ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000529 --> ing12_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000529 --> ing13_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing14_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000529 --> ing14_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing14_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing15_H3BO3["H3BO3"]
    CultureMech_000529 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000529 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_000529 --> ing17__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing17__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000529 --> ing18_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing19_Na2SeO4["Na2SeO4"]
    CultureMech_000529 --> ing19_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing19_Na2SeO4 -.-> CHEBI_77775
    ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000529 --> ing20_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing21_D_____biotin["D-(+)-biotin"]
    CultureMech_000529 --> ing21_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_D_____biotin -.-> CHEBI_15956
    ing22_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000529 --> ing22_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing22_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (23)

Preparation

1. Dissolve ingredients (except bicarbonate, coenzyme M, vitamins, thiosulfate, and dithiothreitol), then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic serum vials (e.g., Balch-type tubes) to 30% of their volume and autoclave. Add coenzyme M, vitamins, thiosulfate, and dithiothreitol from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The vitamin and dithiothreitol solutions should be sterilized by filtration. Adjust the pH of the complete medium to 6.5.
2. After inoculation pressurize culture vessels to 1.5 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
3. First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Solutions

Target organisms

No target organisms are recorded.

Provenance