medium_for_enhydromyxa_salsuginosa

CultureMech:000536 bacterial COMPLEX SOLID_AGAR
Original name: MEDIUM FOR ENHYDROMYXA SALSUGINOSA

Overview

External ontology
mediadive.medium:1102 (source link unavailable) — MEDIUM FOR ENHYDROMYXA SALSUGINOSA
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1102.pdf

Composition

flowchart LR
    CultureMech_000536["medium_for_enhydromyxa_salsuginosa"]
    ing0_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_000536 --> ing0_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing0_Fe_III__citrate -.-> CHEBI_144421
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000536 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000536 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_000536 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaCl["NaCl"]
    CultureMech_000536 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Na_caseinate["Na-caseinate"]
    CultureMech_000536 --> ing5_Na_caseinate
    ing6_HEPES["HEPES"]
    CultureMech_000536 --> ing6_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing6_HEPES -.-> CHEBI_46756
    ing7_NaHCO3["NaHCO3"]
    CultureMech_000536 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_H3BO3["H3BO3"]
    CultureMech_000536 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_KBr["KBr"]
    CultureMech_000536 --> ing9_KBr
    CHEBI_32030["CHEBI:32030"]
    ing9_KBr -.-> CHEBI_32030
    ing10_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_000536 --> ing10_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing10_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing11_Agar["Agar"]
    CultureMech_000536 --> ing11_Agar
    CHEBI_2509["CHEBI:2509"]
    ing11_Agar -.-> CHEBI_2509
    ing12_Vitamin_B12["Vitamin B12"]
    CultureMech_000536 --> ing12_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing12_Vitamin_B12 -.-> CHEBI_176843
    ing13_Na2_EDTA["Na2-EDTA"]
    CultureMech_000536 --> ing13_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing13_Na2_EDTA -.-> CHEBI_64734
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000536 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000536 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000536 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000536 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000536 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000536 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000536 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 Fe(III) citrate
C6H5FeO7 244.944 g/mol
0.02 G_PER_L CHEBI:144421
iron(III) citrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.6 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
5 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
6 Na-caseinate 0.5 G_PER_L
7 HEPES 2 G_PER_L CHEBI:46756
HEPES
8 NaHCO3
CHO3.Na 84.006 g/mol
0.16 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 H3BO3
H3BO3 61.833 g/mol
0.32 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.02, 0.3]
10 KBr
Br.K 119.002 g/mol
0.08 G_PER_L CHEBI:32030
potassium bromide
11 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.03 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
12 Agar 20 G_PER_L CHEBI:2509
agar
13 Vitamin B12 0.0005 G_PER_L CHEBI:176843
vitamin B12
14 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
17 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust to pH 7.2, check and adjust pH after autoclaving. Add vitamin B12 from a filter sterilized stock solution after autoclaving.
  2. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.386303Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1102
2026-03-13T03:08:38.220907Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:56.185220Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000536
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.233369+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR ENHYDROMYXA SALSUGINOSA" to "medium_for_enhydromyxa_salsuginosa" for consistent matching
2026-06-05T07:41:29.042475+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.050381+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.