nitriliruptor_alkaliphilus_medium

CultureMech:000538 bacterial DEFINED LIQUID
Original name: NITRILIRUPTOR ALKALIPHILUS MEDIUM

Overview

External ontology
mediadive.medium:1105 (source link unavailable) — NITRILIRUPTOR ALKALIPHILUS MEDIUM
Applications
  • Microbial cultivation
pH
10.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1105.pdf

Composition

flowchart LR
    CultureMech_000538["nitriliruptor_alkaliphilus_medium"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_000538 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_000538 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NaCl["NaCl"]
    CultureMech_000538 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000538 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000538 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000538 --> ing5_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing6_Vitamin_B12["Vitamin B12"]
    CultureMech_000538 --> ing6_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing6_Vitamin_B12 -.-> CHEBI_176843
    ing7_Isobutyramide["Isobutyramide"]
    CultureMech_000538 --> ing7_Isobutyramide
    CHEBI_193555["CHEBI:193555"]
    ing7_Isobutyramide -.-> CHEBI_193555
    ing8_Na2S2O3["Na2S2O3"]
    CultureMech_000538 --> ing8_Na2S2O3
    CHEBI_132112["CHEBI:132112"]
    ing8_Na2S2O3 -.-> CHEBI_132112
    ing9_EDTA["EDTA"]
    CultureMech_000538 --> ing9_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing9_EDTA -.-> CHEBI_4735
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000538 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_ZnSO4["ZnSO4"]
    CultureMech_000538 --> ing11_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing11_ZnSO4 -.-> CHEBI_35176
    ing12_MnCl2["MnCl2"]
    CultureMech_000538 --> ing12_MnCl2
    CHEBI_63041["CHEBI:63041"]
    ing12_MnCl2 -.-> CHEBI_63041
    ing13_H3BO3["H3BO3"]
    CultureMech_000538 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000538 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2["CuCl2"]
    CultureMech_000538 --> ing15_CuCl2
    CHEBI_49553["CHEBI:49553"]
    ing15_CuCl2 -.-> CHEBI_49553
    ing16_NiCl2_x_2_H2O["NiCl2 x 2 H2O"]
    CultureMech_000538 --> ing16_NiCl2_x_2_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_2_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000538 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Na2CO3
CO3.2Na 105.988 g/mol
22 G_PER_L CHEBI:29377
sodium carbonate
2 NaHCO3
CHO3.Na 84.006 g/mol
8 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 NaCl
Cl.Na 58.443 g/mol
6 G_PER_L CHEBI:26710
sodium chloride
4 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
10 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
7 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
8 Isobutyramide
C4H9NO 87.122 g/mol
0.8712 G_PER_L CHEBI:193555
isobutyramide
9 Na2S2O3
2Na.O3S2 158.111 g/mol
500 G_PER_L CHEBI:132112
sodium thiosulfate
10 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
11 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
12 ZnSO4
O4S.Zn 161.453 g/mol
0.1 G_PER_L CHEBI:35176
zinc sulfate
13 MnCl2
Cl2Mn 125.844 g/mol
0.03 G_PER_L CHEBI:63041
manganese(II) chloride
14 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2
Cl2Cu 134.452 g/mol
0.01 G_PER_L CHEBI:49553
copper(II) chloride
17 NiCl2 x 2 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Dissolve in this order in 1L preferably MilliQ water. Sterilize in closed bottles at 120°C 20 min. After cooling, usually there will be some precipitate on the bottom. That´s why it is handy to have another empty 1L bottle to decant clear top solution into it.
  2. After sterilization add from concentrated steril stock solutions:
  3. Trace metals, MgSO4 x 7 H2O and Na2S2O3 x 5 H2O solution you can autoclave
  4. Vitamin B12 and Isobutyramide solution you have to filter-sterilize
  5. 50% solution of Na2S2O3 (Fluka), pH 9-10 >> filter sterilize or 110°C 20 min
  6. pH final 3-4 by HCl

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.395547Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1105
2026-02-06T05:10:41.629971Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:56.210303Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000538
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.261547+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NITRILIRUPTOR ALKALIPHILUS MEDIUM" to "nitriliruptor_alkaliphilus_medium" for consistent matching
2026-06-05T07:41:39.731575+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.695735+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:09.570005+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-20T19:03:00.078079+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).