sporosalibacterium_medium

CultureMech:000539 bacterial COMPLEX LIQUID
Original name: SPOROSALIBACTERIUM MEDIUM

Overview

External ontology
mediadive.medium:1106 (source link unavailable) — SPOROSALIBACTERIUM MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1106.pdf

Composition

flowchart LR
    CultureMech_000539["sporosalibacterium_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000539 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000539 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KCl["KCl"]
    CultureMech_000539 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000539 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_000539 --> ing4_Yeast_extract
    ing5_Trypticase_peptone["Trypticase peptone"]
    CultureMech_000539 --> ing5_Trypticase_peptone
    ing6_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000539 --> ing6_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Sodium_resazurin -.-> CHEBI_8806
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000539 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_Na2CO3["Na2CO3"]
    CultureMech_000539 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_D_Glucose["D-Glucose"]
    CultureMech_000539 --> ing9_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing9_D_Glucose -.-> CHEBI_17634
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000539 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000539 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
40 G_PER_L CHEBI:26710
sodium chloride
2 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
3 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 Yeast extract 2 G_PER_L
6 Trypticase peptone 2 G_PER_L
7 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
3 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 Na2CO3
CO3.2Na 105.988 g/mol
1 G_PER_L CHEBI:29377
sodium carbonate
10 D-Glucose
C6H12O6 180.156 g/mol
3.6 G_PER_L CHEBI:17634
D-glucose
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Dissolve ingredients (except magnesium chloride, carbonate, glucose and reducing agents) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 -45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add magnesium chloride, glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The glucose solution should be sterilized by filtration. Adjust pH of complete medium to 6.8 - 7.0, if necessary.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.398705Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1106
2026-03-13T06:18:56.217697Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000539
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.269953+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SPOROSALIBACTERIUM MEDIUM" to "sporosalibacterium_medium" for consistent matching
2026-05-20T06:02:05.929208+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:41:46.788253+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.760841+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.