Overview
- External ontology
- mediadive.medium:1107 (source link unavailable) — DIALISTER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1107.pdf
Composition
flowchart LR
CultureMech_000540["dialister_medium"]
ing0_Na_L_lactate["Na-L-lactate"]
CultureMech_000540 --> ing0_Na_L_lactate
CHEBI_232798["CHEBI:232798"]
ing0_Na_L_lactate -.-> CHEBI_232798
ing1_Haemin["Haemin"]
CultureMech_000540 --> ing1_Haemin
CHEBI_50385["CHEBI:50385"]
ing1_Haemin -.-> CHEBI_50385
ing2_NaHCO3["NaHCO3"]
CultureMech_000540 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_Glucose["Glucose"]
CultureMech_000540 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Cystein_HCl_x_2_H2O["Cystein-HCl x 2 H2O"]
CultureMech_000540 --> ing4_Cystein_HCl_x_2_H2O
ing5_DTT["DTT"]
CultureMech_000540 --> ing5_DTT
CHEBI_18320["CHEBI:18320"]
ing5_DTT -.-> CHEBI_18320
ing6_Na_formate["Na-formate"]
CultureMech_000540 --> ing6_Na_formate
CHEBI_62965["CHEBI:62965"]
ing6_Na_formate -.-> CHEBI_62965
ing7_Na_fumarate["Na-fumarate"]
CultureMech_000540 --> ing7_Na_fumarate
CHEBI_115156["CHEBI:115156"]
ing7_Na_fumarate -.-> CHEBI_115156
ing8_Horse_blood["Horse blood"]
CultureMech_000540 --> ing8_Horse_blood
ing9_Trypticase["Trypticase"]
CultureMech_000540 --> ing9_Trypticase
ing10_Yeast_extract["Yeast extract"]
CultureMech_000540 --> ing10_Yeast_extract
ing11_NaCl["NaCl"]
CultureMech_000540 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_KNO3["KNO3"]
CultureMech_000540 --> ing12_KNO3
CHEBI_63043["CHEBI:63043"]
ing12_KNO3 -.-> CHEBI_63043
ing13_Vitamin_K1["Vitamin K1"]
CultureMech_000540 --> ing13_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing13_Vitamin_K1 -.-> CHEBI_18067
ing14_Ethanol["Ethanol"]
CultureMech_000540 --> ing14_Ethanol
CHEBI_16236["CHEBI:16236"]
ing14_Ethanol -.-> CHEBI_16236
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na-L-lactate
C3H5O3.Na 112.06 g/mol
|
0.05 G_PER_L |
CHEBI:232798
sodium L-lactate
|
|
| 2 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
0.1 G_PER_L |
CHEBI:50385
hemin
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.01 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.04 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Cystein-HCl x 2 H2O | 0.0025 G_PER_L | ||
| 6 |
DTT
C4H10O2S2 154.256 g/mol
|
0.000771255 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 7 |
Na-formate
CHO2.Na 68.007 g/mol
|
0.03 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 8 |
Na-fumarate
C4H2O4.2Na 160.036 g/mol
|
0.03 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 9 | Horse blood | 0.5 G_PER_L | ||
| 10 | Trypticase | 17 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 11 | Yeast extract | 3 G_PER_L | ||
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
3 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 13 |
KNO3
K.NO3 101.102 g/mol
|
3 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 14 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.1 G_PER_L |
CHEBI:18067
phylloquinone
|
|
| 15 |
Ethanol
C2H6O 46.069 g/mol
|
19 G_PER_L |
CHEBI:16236
ethanol
|
Preparation
- To 10 ml of the dialister medium add aseptically:
- Filter-sterilize all solutions and incubate them in oxygen-free atmosphere.
- Adjust pH to 7.0.
- Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere.
- Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.400955Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1107 |
| 2026-01-28T19:48:07.743902Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:56.224961Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000540 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.277792+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DIALISTER MEDIUM" to "dialister_medium" for consistent matching |
| 2026-06-05T07:40:58.650832+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.728616+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:56.357552+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/9/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |