Overview
- External ontology
- mediadive.medium:1108a (source link unavailable) — CYS MEDIUM WITH MODIFIED NACL CONCENTRATION
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1108a.pdf
Composition
flowchart LR
CultureMech_000542["cys_medium_with_modified_nacl_concentration"]
ing0_N_Z_Case["N-Z-Case"]
CultureMech_000542 --> ing0_N_Z_Case
ing1_Yeast_extract["Yeast extract"]
CultureMech_000542 --> ing1_Yeast_extract
ing2_Starch["Starch"]
CultureMech_000542 --> ing2_Starch
CHEBI_28017["CHEBI:28017"]
ing2_Starch -.-> CHEBI_28017
ing3_NaCl["NaCl"]
CultureMech_000542 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2["MgCl2"]
CultureMech_000542 --> ing4_MgCl2
CHEBI_6636["CHEBI:6636"]
ing4_MgCl2 -.-> CHEBI_6636
ing5_CaCl2["CaCl2"]
CultureMech_000542 --> ing5_CaCl2
CHEBI_3312["CHEBI:3312"]
ing5_CaCl2 -.-> CHEBI_3312
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000542 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_Gelrite["Gelrite"]
CultureMech_000542 --> ing7_Gelrite
CHEBI_85248["CHEBI:85248"]
ing7_Gelrite -.-> CHEBI_85248
ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000542 --> ing8_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing9_VOSO4_x_H2O["VOSO4 x H2O"]
CultureMech_000542 --> ing9_VOSO4_x_H2O
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000542 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000542 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000542 --> ing12_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000542 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000542 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | N-Z-Case | 3 G_PER_L | ||
| 2 | Yeast extract | 2 G_PER_L | ||
| 3 | Starch | 1 G_PER_L |
CHEBI:28017
starch
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgCl2
2Cl.Mg 95.211 g/mol
|
0.125 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 6 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.025 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 | Gelrite | 8 G_PER_L |
CHEBI:85248
gellan gum
|
|
| 9 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
12 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 10 | VOSO4 x H2O | 1 G_PER_L | ||
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
5 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.6 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.15 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
8 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Preparation
- Add 100 µl each of Trace Metal solutions according to the following order:
- Adjust to pH 7.5 at room temperature and add water to the final volume of 1 liter. Sterilize with and autoclave at 121°C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.405784Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1108a |
| 2026-03-13T06:18:56.239447Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000542 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.294646+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CYS MEDIUM WITH MODIFIED NACL CONCENTRATION" to "cys_medium_with_modified_nacl_concentration" for consistent matching |
| 2026-06-05T07:40:55.216484+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |