cmc_medium_n2_co2

Overview

External ontology

mediadive.medium:110a (source link unavailable) — CMC MEDIUM (N2/CO2)

Applications

• Microbial cultivation

pH

7.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium110a.pdf

Composition

flowchart LR
    CultureMech_000545["cmc_medium_n2_co2"]
    ing0_Casitone["Casitone"]
    CultureMech_000545 --> ing0_Casitone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000545 --> ing1_Yeast_extract
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000545 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000545 --> ing3_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Sodium_resazurin -.-> CHEBI_8806
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000545 --> ing4_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing5_Na2CO3["Na2CO3"]
    CultureMech_000545 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_Ground_beef["Ground beef"]
    CultureMech_000545 --> ing6_Ground_beef
    ing7_NaOH["NaOH"]
    CultureMech_000545 --> ing7_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing7_NaOH -.-> CHEBI_32145
    ing8_D_Glucose["D-Glucose"]
    CultureMech_000545 --> ing8_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing8_D_Glucose -.-> CHEBI_17634
    ing9_Cellobiose["Cellobiose"]
    CultureMech_000545 --> ing9_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing9_Cellobiose -.-> CHEBI_17057
    ing10_D_Maltose["D-Maltose"]
    CultureMech_000545 --> ing10_D_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing10_D_Maltose -.-> CHEBI_17306
    ing11_Starch["Starch"]
    CultureMech_000545 --> ing11_Starch
    CHEBI_28017["CHEBI:28017"]
    ing11_Starch -.-> CHEBI_28017

Ingredients (12)

Preparation

1. Dissolve ingredients, except cysteine, sugar mix, and carbonate, boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere, add 0.5 g/l L-cysteine-HCl x H2O and dispense under same gas atmosphere into Hungate-type tubes (for strains demanding meat particles put those first into the tube, use 1 part meat particles to 4 or 5 parts fluid). Autoclave at 121°C for 20 min. After autoclaving add the sugar mix from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust the pH of the medium to pH 7.0, if necessary.
2. Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000.0 ml.

Target organisms

No target organisms are recorded.

Provenance