Overview
- External ontology
- mediadive.medium:1113 (source link unavailable) — LEPTOSPIRA-MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1113.pdf
Composition
flowchart LR
CultureMech_000549["leptospira_medium"]
ing0_Leptospira_Medium_Base_EMJH["Leptospira Medium Base EMJH"]
CultureMech_000549 --> ing0_Leptospira_Medium_Base_EMJH
ing1_Agarose["Agarose"]
CultureMech_000549 --> ing1_Agarose
CHEBI_2511["CHEBI:2511"]
ing1_Agarose -.-> CHEBI_2511
ing2_Leptospira_Enrichment_EMJH["Leptospira Enrichment EMJH"]
CultureMech_000549 --> ing2_Leptospira_Enrichment_EMJH
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Leptospira Medium Base EMJH | 2.3 G_PER_L | ||
| 2 |
Agarose
(C12H18O9)n.C12H20O10 630.549 g/mol
|
1.5 G_PER_L |
CHEBI:2511
agarose
|
|
| 3 | Leptospira Enrichment EMJH | 100 G_PER_L |
Preparation
- Dissolve the agarose in water by boiling. Add the Leptospira base and dissolve completely. Autoclave at 121°C for 15 min. Allow the medium to cool to 50-55°C. Warm the Leptospira Enrichment to approximately 25°C and add to the warm medium. Mix uniformly and dispense aseptically into sterile tubes as requested. Final pH is 7.5 at 25°C.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.420464Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1113 |
| 2026-03-13T06:18:56.296779Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000549 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.358462+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LEPTOSPIRA-MEDIUM" to "leptospira_medium" for consistent matching |
| 2026-06-05T07:41:11.099876+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-13T00:00:00-07:00 | repair_remaining_score10_grounding_batch9.py | RESOLVED_REMAINING_SCORE10_GROUNDING_BATCH9 | Reviewed remaining score-10 grounding rows: Leptospira Medium Base EMJH, Leptospira Enrichment EMJH. |