Overview
- External ontology
- mediadive.medium:1122 (source link unavailable) — MEDIUM K
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1122.pdf
Composition
flowchart LR
CultureMech_000559["medium_k"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_000559 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_000559 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NaCl["NaCl"]
CultureMech_000559 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000559 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000559 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_Agar["Agar"]
CultureMech_000559 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Methanol["Methanol"]
CultureMech_000559 --> ing6_Methanol
CHEBI_17790["CHEBI:17790"]
ing6_Methanol -.-> CHEBI_17790
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.125 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.002 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
Methanol
CH4O 32.042 g/mol
|
1 G_PER_L |
CHEBI:17790
methanol
|
Preparation
- Adjust to pH 7.2.
- After autoclaving add methanol (sterilized separately) to a concentration of 1% (v/v).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.448030Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1122 |
| 2026-03-13T06:18:56.387885Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000559 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.466003+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM K" to "medium_k" for consistent matching |
| 2026-06-05T07:41:30.190044+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.474459+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |