Overview
- External ontology
- mediadive.medium:1130 (source link unavailable) — RE-101/102 MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.2}
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1130.pdf
Composition
flowchart LR
CultureMech_000566["re_101_102_medium"]
ing0_NaCl["NaCl"]
CultureMech_000566 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000566 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_K2SO4["K2SO4"]
CultureMech_000566 --> ing2_K2SO4
CHEBI_32036["CHEBI:32036"]
ing2_K2SO4 -.-> CHEBI_32036
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000566 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Yeast_extract["Yeast extract"]
CultureMech_000566 --> ing4_Yeast_extract
ing5_Agar["Agar"]
CultureMech_000566 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
175 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 | Yeast extract | 5 G_PER_L | ||
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient |
Preparation
- Adjust to pH 6.8-7.2
- Note: for Agar plates, autoclave NaCl separately. Otherwise the salts will precipitate and the plates not solidify properly.
- Note: especially for strains DSM 17983 and 17984, yeast extract from ThermoFisher has to be used. Yeast extract from other vendors might not support growth.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.466060Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1130 |
| 2026-03-13T06:18:56.423117Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000566 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.507144+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RE-101/102 MEDIUM" to "re_101_102_medium" for consistent matching |
| 2026-05-20T06:02:03.956660+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7.2' -> {'min': 6.8, 'max': 7.2} |
| 2026-06-05T07:41:44.046778+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.868297+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |