lc_2

CultureMech:000568 bacterial DEFINED LIQUID
Original name: LC 2

Overview

External ontology
mediadive.medium:1132 (source link unavailable) — LC 2
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1132.pdf

Composition

flowchart LR
    CultureMech_000568["lc_2"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000568 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000568 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_KNO3["KNO3"]
    CultureMech_000568 --> ing2_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing2_KNO3 -.-> CHEBI_63043
    ing3_HEPES_buffer["HEPES buffer"]
    CultureMech_000568 --> ing3_HEPES_buffer
    CHEBI_46756["CHEBI:46756"]
    ing3_HEPES_buffer -.-> CHEBI_46756
    ing4_Fe_III_NH4_EDTA["Fe(III)NH4-EDTA"]
    CultureMech_000568 --> ing4_Fe_III_NH4_EDTA
    ing5_Methanol["Methanol"]
    CultureMech_000568 --> ing5_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing5_Methanol -.-> CHEBI_17790
    ing6_HCl["HCl"]
    CultureMech_000568 --> ing6_HCl
    CHEBI_17883["CHEBI:17883"]
    ing6_HCl -.-> CHEBI_17883
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000568 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_ZnCl2["ZnCl2"]
    CultureMech_000568 --> ing8_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing8_ZnCl2 -.-> CHEBI_49976
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000568 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_000568 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000568 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000568 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000568 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000568 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_Na2HPO4["Na2HPO4"]
    CultureMech_000568 --> ing15_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing15_Na2HPO4 -.-> CHEBI_34683
    ing16_KH2PO4["KH2PO4"]
    CultureMech_000568 --> ing16_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing16_KH2PO4 -.-> CHEBI_63036

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 KNO3
K.NO3 101.102 g/mol
0.05 G_PER_L CHEBI:63043
potassium nitrate
4 HEPES buffer 0.4766 G_PER_L CHEBI:46756
HEPES
5 Fe(III)NH4-EDTA 1 G_PER_L
6 Methanol
CH4O 32.042 g/mol
0.2376 G_PER_L CHEBI:17790
methanol
7 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
8 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
9 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 Na2HPO4
HO4P.2Na 141.958 g/mol
36 G_PER_L CHEBI:34683
disodium hydrogenphosphate
17 KH2PO4
H2O4P.K 136.084 g/mol
14 G_PER_L CHEBI:63036
potassium dihydrogen phosphate

Preparation

  1. Dispense 5 ml aliquots into Hungate tubes. After autoclaving add the following components per liter:
  2. Adjust to pH 6.8.
  3. Incubate in sealed containers. The strain held in the DSMZ has been cultivated on methanol, but the original publication indicates that the strain will also grow on methane.
  4. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-26T17:53:13.472916Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1132
2026-03-13T06:18:56.447063Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000568
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.533533+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LC 2" to "lc_2" for consistent matching
2026-06-05T07:41:10.968646+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.042150+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:00.495554+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.