rubritalea_medium

CultureMech:000573 bacterial COMPLEX LIQUID
Original name: RUBRITALEA MEDIUM

Overview

External ontology
mediadive.medium:1137 (source link unavailable) — RUBRITALEA MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1137.pdf

Composition

flowchart LR
    CultureMech_000573["rubritalea_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_000573 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000573 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_000573 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Tris_HCl["Tris-HCl"]
    CultureMech_000573 --> ing3_Tris_HCl
    ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000573 --> ing4_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000573 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000573 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000573 --> ing7__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing7__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000573 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_Na_EDTA["Na-EDTA"]
    CultureMech_000573 --> ing9_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na_EDTA -.-> CHEBI_64734
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000573 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000573 --> ing11_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing11_MnSO4_x_H2O -.-> CHEBI_86364
    ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000573 --> ing12_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing13_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000573 --> ing13_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing13_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing14_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_000573 --> ing14_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing14_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing15_NaCl["NaCl"]
    CultureMech_000573 --> ing15_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing15_NaCl -.-> CHEBI_26710
    ing16_Na2SO4["Na2SO4"]
    CultureMech_000573 --> ing16_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing16_Na2SO4 -.-> CHEBI_32149
    ing17_NaHCO3["NaHCO3"]
    CultureMech_000573 --> ing17_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing17_NaHCO3 -.-> CHEBI_32139
    ing18_KCl["KCl"]
    CultureMech_000573 --> ing18_KCl
    CHEBI_32588["CHEBI:32588"]
    ing18_KCl -.-> CHEBI_32588
    ing19_KBr["KBr"]
    CultureMech_000573 --> ing19_KBr
    CHEBI_32030["CHEBI:32030"]
    ing19_KBr -.-> CHEBI_32030
    ing20_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000573 --> ing20_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing20_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing21_H3BO3["H3BO3"]
    CultureMech_000573 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Peptone 1.5 G_PER_L
2 Yeast extract 1.5 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
1.5 G_PER_L CHEBI:17234
glucose
4 Tris-HCl 5 G_PER_L
5 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
7.685 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 3.335, 4.35]
8 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
10 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
13 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
14 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
15 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
16 NaCl
Cl.Na 58.443 g/mol
70.43 G_PER_L CHEBI:26710
sodium chloride
17 Na2SO4
2Na.O4S 142.043 g/mol
11.75 G_PER_L CHEBI:32149
sodium sulfate
18 NaHCO3
CHO3.Na 84.006 g/mol
2.88 G_PER_L CHEBI:32139
sodium hydrogencarbonate
19 KCl
Cl.K 74.551 g/mol
1.99 G_PER_L CHEBI:32588
potassium chloride
20 KBr
Br.K 119.002 g/mol
0.29 G_PER_L CHEBI:32030
potassium bromide
21 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
31.86 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
22 H3BO3
H3BO3 61.833 g/mol
0.08 G_PER_L CHEBI:33118
boric acid

Preparation

  1. Adjust to pH 7.5
  2. Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
  3. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.
  4. Add NaHCO3 from a filter-sterilized stock solution when the medium has cooled.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.484246Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1137
2026-02-06T05:10:45.150065Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:29.540392Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:56.481056Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000573
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.572849+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RUBRITALEA MEDIUM" to "rubritalea_medium" for consistent matching
2026-06-05T07:41:44.746297+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.108142+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.