sulfurimonas_hongkongensis_medium

CultureMech:000577 bacterial DEFINED LIQUID
Original name: SULFURIMONAS HONGKONGENSIS MEDIUM

Overview

External ontology
mediadive.medium:113a (source link unavailable) — SULFURIMONAS HONGKONGENSIS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.2}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium113a.pdf

Composition

flowchart LR
    CultureMech_000577["sulfurimonas_hongkongensis_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000577 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000577 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_KNO3["KNO3"]
    CultureMech_000577 --> ing2_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing2_KNO3 -.-> CHEBI_63043
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000577 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000577 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000577 --> ing5_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing6_Na2CO3["Na2CO3"]
    CultureMech_000577 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000577 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_H2SO4["H2SO4"]
    CultureMech_000577 --> ing8_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing8_H2SO4 -.-> CHEBI_26836
    ing9_Na2_EDTA["Na2-EDTA"]
    CultureMech_000577 --> ing9_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na2_EDTA -.-> CHEBI_64734
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000577 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000577 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_000577 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000577 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000577 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000577 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000577 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_Biotin["Biotin"]
    CultureMech_000577 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Folic_acid["Folic acid"]
    CultureMech_000577 --> ing18_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing18_Folic_acid -.-> CHEBI_27470
    ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000577 --> ing19_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing20_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000577 --> ing20_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing20_Thiamine_HCl -.-> CHEBI_49105
    ing21_Riboflavin["Riboflavin"]
    CultureMech_000577 --> ing21_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavin -.-> CHEBI_17015
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000577 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000577 --> ing23_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_000577 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000577 --> ing25_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing26__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000577 --> ing26__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing26__DL__alpha_Lipoic_acid -.-> CHEBI_16494

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
26.5393 G_PER_L CHEBI:26710
sodium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
2.12314 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 KNO3
K.NO3 101.102 g/mol
2.12314 G_PER_L CHEBI:63043
potassium nitrate
4 NH4Cl
Cl.H4N 53.492 g/mol
1.06157 G_PER_L CHEBI:31206
ammonium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.849257 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
125 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
7 Na2CO3
CO3.2Na 105.988 g/mol
50 G_PER_L CHEBI:29377
sodium carbonate
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 2.0, 0.2]
9 H2SO4
H2O4S 98.079 g/mol
10 G_PER_L CHEBI:26836
sulfuric acid
10 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
19 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
20 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
21 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
22 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
23 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
24 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
25 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
26 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
27 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Solution A is prepared under 100% N2 gas atmosphere and sterilized by autoclaving at 121⁰C for 15 min. Solution C is sterilized by autoclaving under an atmosphere of 80% N2 and 20% CO2 gas mixture. Solutions B, D, and E are prepared under a 100% N2 gas atmosphere and sterilized by filtration. Appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Check the pH of the complete medium and adjust to pH 7.0-7.2, if necessary.
  2. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.195896Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 113a
2026-02-06T05:10:50.687783Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:37.343203Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:56.511586Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000577
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.608652+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SULFURIMONAS HONGKONGENSIS MEDIUM" to "sulfurimonas_hongkongensis_medium" for consistent matching
2026-05-20T06:02:06.657317+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2}
2026-06-05T07:41:47.770257+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.888695+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:12.157248+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.