Overview
- External ontology
- mediadive.medium:1144 (source link unavailable) — SINGULISPHAERA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1144.pdf
Composition
flowchart LR
CultureMech_000582["singulisphaera_medium"]
ing0_N_Acetylglucosamine["N-Acetylglucosamine"]
CultureMech_000582 --> ing0_N_Acetylglucosamine
CHEBI_59640["CHEBI:59640"]
ing0_N_Acetylglucosamine -.-> CHEBI_59640
ing1_KH2PO4["KH2PO4"]
CultureMech_000582 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Na_ampicillin["Na-ampicillin"]
CultureMech_000582 --> ing2_Na_ampicillin
CHEBI_34535["CHEBI:34535"]
ing2_Na_ampicillin -.-> CHEBI_34535
ing3_Peptone["Peptone"]
CultureMech_000582 --> ing3_Peptone
ing4_Yeast_extract["Yeast extract"]
CultureMech_000582 --> ing4_Yeast_extract
ing5_Agar["Agar"]
CultureMech_000582 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000582 --> ing6_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000582 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000582 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_000582 --> ing9__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing9__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000582 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_Na_EDTA["Na-EDTA"]
CultureMech_000582 --> ing11_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing11_Na_EDTA -.-> CHEBI_64734
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000582 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000582 --> ing13_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing13_MnSO4_x_H2O -.-> CHEBI_86364
ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000582 --> ing14_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
ing15_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_000582 --> ing15_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing15_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
ing16_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_000582 --> ing16_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing16_Na2B4O7_x_10_H2O -.-> CHEBI_131366
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | N-Acetylglucosamine | 1 G_PER_L |
CHEBI:59640
N-acetylglucosamine
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Na-ampicillin
C16H18N3O4S.Na 371.394 g/mol
|
0.2 G_PER_L |
CHEBI:34535
ampicillin sodium
|
|
| 4 | Peptone | 0.1 G_PER_L | ||
| 5 | Yeast extract | 0.1 G_PER_L | ||
| 6 | Agar | 18 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.00925 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.599 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.099, 0.5] |
| 12 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.154 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 15 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0392 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 16 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.0248 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
|
| 17 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0177 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Preparation
- Adjust pH to 5.8.
- Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
- Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.497809Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1144 |
| 2026-03-13T03:09:27.155235Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:56.558983Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000582 |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.639528+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SINGULISPHAERA MEDIUM" to "singulisphaera_medium" for consistent matching |
| 2026-06-05T07:41:45.868345+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.497137+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |