ms_medium_modified

CultureMech:000583 bacterial DEFINED LIQUID
Original name: MS-MEDIUM (MODIFIED)

Overview

External ontology
mediadive.medium:1145 (source link unavailable) — MS-MEDIUM (MODIFIED)
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1145.pdf

Composition

flowchart LR
    CultureMech_000583["ms_medium_modified"]
    ing0_Double_distilled_water["Double distilled water"]
    CultureMech_000583 --> ing0_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Double_distilled_water -.-> CHEBI_15377
    ing1_Elemental_sulphur["Elemental sulphur"]
    CultureMech_000583 --> ing1_Elemental_sulphur
    CHEBI_33403["CHEBI:33403"]
    ing1_Elemental_sulphur -.-> CHEBI_33403
    ing2_NaS2O3_x_5_H2O["NaS2O3 x 5 H2O"]
    CultureMech_000583 --> ing2_NaS2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing2_NaS2O3_x_5_H2O -.-> CHEBI_32150
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000583 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_KCl["KCl"]
    CultureMech_000583 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000583 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000583 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_NaOH["NaOH"]
    CultureMech_000583 --> ing7_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing7_NaOH -.-> CHEBI_32145
    ing8_NH4Cl["NH4Cl"]
    CultureMech_000583 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000583 --> ing9_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing10_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_000583 --> ing10_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing10_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing11_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000583 --> ing11_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing11_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000583 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000583 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000583 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
    CultureMech_000583 --> ing15_NiSO4_x_6_H2O
    CHEBI_53001["CHEBI:53001"]
    ing15_NiSO4_x_6_H2O -.-> CHEBI_53001
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000583 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_H2SeO3["H2SeO3"]
    CultureMech_000583 --> ing17_H2SeO3
    CHEBI_26642["CHEBI:26642"]
    ing17_H2SeO3 -.-> CHEBI_26642
    ing18_ZnCl2["ZnCl2"]
    CultureMech_000583 --> ing18_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing18_ZnCl2 -.-> CHEBI_49976
    ing19_H3BO3["H3BO3"]
    CultureMech_000583 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
    CultureMech_000583 --> ing20_AlCl3_x_6_H2O
    CHEBI_30115["CHEBI:30115"]
    ing20_AlCl3_x_6_H2O -.-> CHEBI_30115
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000583 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Double distilled water
H2O 18.015 g/mol
800 G_PER_L CHEBI:15377
water
2 Elemental sulphur 5 G_PER_L CHEBI:33403
elemental sulfur
3 NaS2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
2 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 KCl
Cl.K 74.551 g/mol
0.48 G_PER_L CHEBI:32588
potassium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
40.4 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.4, 40.0]
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
100.8 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 [Merged 2 duplicates: 0.8, 100.0]
8 NaOH
HO.Na 39.997 g/mol
3.9997 G_PER_L CHEBI:32145
sodium hydroxide
9 NH4Cl
Cl.H4N 53.492 g/mol
100 G_PER_L CHEBI:31206
ammonium chloride
10 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
200 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
11 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
0.5 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
12 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.03 G_PER_L CHEBI:63939
sodium tungstate dihydrate
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.15 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.02 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
0.02 G_PER_L CHEBI:53001
nickel sulfate
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
18 H2SeO3
H2O3Se 128.973 g/mol
0.01 G_PER_L CHEBI:26642
selenous acid
19 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
0.04 G_PER_L CHEBI:30115
aluminium trichloride hexahydrate
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Each of the solutions (A, B, and C) is prepared with anaerobic double distilled water.
  2. The medium is prepared anaerobically under nitrogen gas and dispensed into Hungate tubes or serum bottle sealed with rubber septa. The medium to gas phase ratio should be about 1:10 (v:v). Sterilise the medium by heating at 100° C for 3 hours on three consecutive days (e.g., in a water bath).
  3. Add 4% O2 for microaerophilic conditions.
  4. 100 mM NaOH saturated with CO2
  5. Adjust to pH 4 with HCl
  6. Adjust pH to 3.0 with HCl

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.500577Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1145
2026-01-28T19:48:25.384582Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:12.037956Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:56.569686Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000583
2026-03-17T06:30:19.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.651077+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MS-MEDIUM (MODIFIED)" to "ms_medium_modified" for consistent matching
2026-06-05T07:41:38.698622+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.218170+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.