Overview
- External ontology
- mediadive.medium:1147 (source link unavailable) — RV5 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1147.pdf
Composition
flowchart LR
CultureMech_000585["rv5_medium"]
ing0_EDTA["EDTA"]
CultureMech_000585 --> ing0_EDTA
CHEBI_4735["CHEBI:4735"]
ing0_EDTA -.-> CHEBI_4735
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000585 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000585 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NaCl["NaCl"]
CultureMech_000585 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_NH4Cl["NH4Cl"]
CultureMech_000585 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_DL_Malic_acid["DL-Malic acid"]
CultureMech_000585 --> ing5_DL_Malic_acid
CHEBI_6650["CHEBI:6650"]
ing5_DL_Malic_acid -.-> CHEBI_6650
ing6_K2HPO4["K2HPO4"]
CultureMech_000585 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_BICINE_buffer["BICINE buffer"]
CultureMech_000585 --> ing7_BICINE_buffer
CHEBI_40957["CHEBI:40957"]
ing7_BICINE_buffer -.-> CHEBI_40957
ing8_Thiamine["Thiamine"]
CultureMech_000585 --> ing8_Thiamine
CHEBI_18385["CHEBI:18385"]
ing8_Thiamine -.-> CHEBI_18385
ing9_Vitamin_B12["Vitamin B12"]
CultureMech_000585 --> ing9_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing9_Vitamin_B12 -.-> CHEBI_176843
ing10_Biotin["Biotin"]
CultureMech_000585 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000585 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000585 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000585 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_H3BO3["H3BO3"]
CultureMech_000585 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000585 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000585 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000585 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_ZnCl2["ZnCl2"]
CultureMech_000585 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
CultureMech_000585 --> ing19_VOSO4_x_2_H2O
CHEBI_87009["CHEBI:87009"]
ing19_VOSO4_x_2_H2O -.-> CHEBI_87009
ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000585 --> ing20_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing21_NaHSeO3["NaHSeO3"]
CultureMech_000585 --> ing21_NaHSeO3
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
EDTA
C10H16N2O8 292.244 g/mol
|
5.21 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
[Merged 2 duplicates: 0.01, 5.2] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.075 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.52 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
DL-Malic acid
C4H6O5 134.087 g/mol
|
4 G_PER_L |
CHEBI:6650
malic acid
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
BICINE buffer
C6H13NO4 163.173 g/mol
|
1.6317 G_PER_L |
CHEBI:40957
N,N-bis(2-hydroxyethyl)glycine
|
|
| 9 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.001 G_PER_L |
CHEBI:18385
thiamine(1+)
|
|
| 10 | Vitamin B12 | 2e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
3e-05 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.017 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.188 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 20 |
VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
|
0.03 G_PER_L |
CHEBI:87009
vanadyl sulfate dihydrate
|
|
| 21 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.002 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 22 | NaHSeO3 | 0.002 G_PER_L |
Preparation
- Filter-sterilize and add after autoclaving:
- The pH of the medium was adjusted to 9.5 by addition of NaOH and then sterilized by autoclaving. Before inoculating medium RV5, 0.1% (w/v) NaHCO3 was added to tubes, bottles, or molten media from a 5% (w/v) filter-sterilized stock solution. For routine culture, medium RV5 supplemented with 0.05% (w/v) Difco yeast extract was used.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.507838Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1147 |
| 2026-03-13T03:08:38.037572Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:56.590473Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000585 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.675091+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RV5 MEDIUM" to "rv5_medium" for consistent matching |
| 2026-06-05T07:41:44.910660+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.172423+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |