oceanithermus_medium

CultureMech:000587 bacterial COMPLEX LIQUID
Original name: OCEANITHERMUS MEDIUM

Overview

External ontology
mediadive.medium:1149 (source link unavailable) — OCEANITHERMUS MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1149.pdf

Composition

flowchart LR
    CultureMech_000587["oceanithermus_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_000587 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000587 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000587 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000587 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_000587 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Na2SO4["Na2SO4"]
    CultureMech_000587 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_NaCl["NaCl"]
    CultureMech_000587 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000587 --> ing7_Yeast_extract
    ing8_NaNO3["NaNO3"]
    CultureMech_000587 --> ing8_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing8_NaNO3 -.-> CHEBI_63005
    ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000587 --> ing9_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing10_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000587 --> ing10_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing10_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000587 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000587 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_ZnCl2["ZnCl2"]
    CultureMech_000587 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000587 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_H3BO3["H3BO3"]
    CultureMech_000587 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000587 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000587 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000587 --> ing18_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing19_Biotin["Biotin"]
    CultureMech_000587 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_000587 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000587 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing22_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000587 --> ing22_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing22_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing23_Riboflavin["Riboflavin"]
    CultureMech_000587 --> ing23_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing23_Riboflavin -.-> CHEBI_17015
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000587 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_000587 --> ing25_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_D_Calcium_pantothenate -.-> CHEBI_31345
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_000587 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000587 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Lipoic_acid["Lipoic acid"]
    CultureMech_000587 --> ing28_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_Lipoic_acid -.-> CHEBI_16494

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.78 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.75 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.36 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Collapsed 2 identical duplicates: 0.1, 0.1]
5 NH4Cl
Cl.H4N 53.492 g/mol
0.53 G_PER_L CHEBI:31206
ammonium chloride
6 Na2SO4
2Na.O4S 142.043 g/mol
0.14 G_PER_L CHEBI:32149
sodium sulfate
7 NaCl
Cl.Na 58.443 g/mol
21.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 20.0, 1.0]
8 Yeast extract 2 G_PER_L
9 NaNO3
NO3.Na 84.994 g/mol
1.7 G_PER_L CHEBI:63005
sodium nitrate
10 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
11 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.35 G_PER_L CHEBI:86254
iron trichloride hexahydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.024 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.025 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.12 G_PER_L CHEBI:34887
nickel dichloride
19 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.026 G_PER_L CHEBI:131361
disodium selenite pentahydrate
20 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
21 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
22 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
23 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
24 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
25 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
26 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
27 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
28 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
29 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Prepare the medium without vitamins, boil and cool under a nitrogen:carbon dioxide (80:20 v/v) atmosphere. Vitamins (filter sterilized and stored under nitrogen) and 100 ml 0.6 M NaHCO3 solution (sterilized and stored under nitrogen) are added from stock solutions.
  2. The pH should be pH 6.5.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.515654Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1149
2026-01-28T19:48:26.270697Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:13.715886Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:56.617189Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000587
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.705195+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "OCEANITHERMUS MEDIUM" to "oceanithermus_medium" for consistent matching
2026-06-05T07:41:40.392186+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.728931+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:38:54.324637+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.