glucose_sulfide_medium_richard_et_al_1985

CultureMech:000593 bacterial DEFINED SOLID_AGAR
Original name: GLUCOSE SULFIDE MEDIUM (Richard et al., 1985)

Overview

External ontology
mediadive.medium:1154 (source link unavailable) — GLUCOSE SULFIDE MEDIUM (Richard et al., 1985)
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1154.pdf

Composition

flowchart LR
    CultureMech_000593["glucose_sulfide_medium_richard_et_al_1985"]
    ing0_Glucose["Glucose"]
    CultureMech_000593 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000593 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_CaCO3["CaCO3"]
    CultureMech_000593 --> ing2_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing2_CaCO3 -.-> CHEBI_3311
    ing3_Ca_NO3_2["Ca(NO3)2"]
    CultureMech_000593 --> ing3_Ca_NO3_2
    CHEBI_64205["CHEBI:64205"]
    ing3_Ca_NO3_2 -.-> CHEBI_64205
    ing4_KCl["KCl"]
    CultureMech_000593 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_K2HPO4["K2HPO4"]
    CultureMech_000593 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000593 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000593 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_Agar["Agar"]
    CultureMech_000593 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Biotin["Biotin"]
    CultureMech_000593 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000593 --> ing10_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing10_Calcium_pantothenate -.-> CHEBI_31345
    ing11_Co_carboxylase["Co-carboxylase"]
    CultureMech_000593 --> ing11_Co_carboxylase
    ing12_Cyanocobalamin["Cyanocobalamin"]
    CultureMech_000593 --> ing12_Cyanocobalamin
    CHEBI_17439["CHEBI:17439"]
    ing12_Cyanocobalamin -.-> CHEBI_17439
    ing13_Folic_acid["Folic acid"]
    CultureMech_000593 --> ing13_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing13_Folic_acid -.-> CHEBI_27470
    ing14_Inositol["Inositol"]
    CultureMech_000593 --> ing14_Inositol
    CHEBI_24848["CHEBI:24848"]
    ing14_Inositol -.-> CHEBI_24848
    ing15_Niacin["Niacin"]
    CultureMech_000593 --> ing15_Niacin
    CHEBI_15940["CHEBI:15940"]
    ing15_Niacin -.-> CHEBI_15940
    ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000593 --> ing16_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing17_Pyridoxine["Pyridoxine"]
    CultureMech_000593 --> ing17_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing17_Pyridoxine -.-> CHEBI_16709
    ing18_Riboflavine["Riboflavine"]
    CultureMech_000593 --> ing18_Riboflavine
    CHEBI_17015["CHEBI:17015"]
    ing18_Riboflavine -.-> CHEBI_17015
    ing19_Thiamine["Thiamine"]
    CultureMech_000593 --> ing19_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing19_Thiamine -.-> CHEBI_18385

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
0.15 G_PER_L CHEBI:17234
glucose
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
3 CaCO3
CO3.Ca 100.086 g/mol
0.1 G_PER_L CHEBI:3311
calcium carbonate
4 Ca(NO3)2
Ca.2NO3 164.086 g/mol
0.1 G_PER_L CHEBI:64205
calcium nitrate
5 KCl
Cl.K 74.551 g/mol
0.05 G_PER_L CHEBI:32588
potassium chloride
6 K2HPO4
HO4P.2K 174.174 g/mol
0.05 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.187 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
9 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
10 Biotin
C10H16N2O3S 244.316 g/mol
6e-06 G_PER_L CHEBI:15956
biotin
11 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
1e-07 G_PER_L CHEBI:31345
Calcium pantothenate
12 Co-carboxylase 1e-07 G_PER_L
13 Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
6e-06 G_PER_L CHEBI:17439
cyanocob(III)alamin
14 Folic acid
C19H19N7O6 441.404 g/mol
6e-06 G_PER_L CHEBI:27470
folic acid
15 Inositol
C6H12O6 180.156 g/mol
1e-07 G_PER_L CHEBI:24848
inositol
16 Niacin
C6H5NO2 123.111 g/mol
1e-07 G_PER_L CHEBI:15940
nicotinic acid
17 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
1e-07 G_PER_L CHEBI:30753
4-aminobenzoic acid
18 Pyridoxine
C8H11NO3 169.18 g/mol
1e-07 G_PER_L CHEBI:16709
pyridoxine
19 Riboflavine
C17H20N4O6 376.369 g/mol
1e-07 G_PER_L CHEBI:17015
riboflavin
20 Thiamine
C12H17N4OS 265.362 g/mol
1e-07 G_PER_L CHEBI:18385
thiamine(1+)

Preparation

  1. Adjust to pH 7.3. Sterilize by autoclaving at 121°C for 15 minutes.
  2. filter-sterilized, added after autoclaving

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.529597Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1154
2026-02-06T05:10:33.468166Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:56.661223Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000593
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.755695+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GLUCOSE SULFIDE MEDIUM (Richard et al., 1985)" to "glucose_sulfide_medium_richard_et_al_1985" for consistent matching
2026-06-05T07:41:06.518966+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.366198+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:59.130690+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.