Overview
- External ontology
- mediadive.medium:1157 (source link unavailable) — PYEM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1157.pdf Stock stages and water inputs are explicit. Percentage bases, stock solvents and measured final volume are not supplied by these sources; no downstream dilution or growth claim is inferred. Existing chemical mappings and high_metal/other historical flags are retained but not validated by this concentration repair.
Composition
flowchart LR
CultureMech_000595["pyem_medium"]
ing0_Peptone["Peptone"]
CultureMech_000595 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_000595 --> ing1_Yeast_extract
ing2_NH4Cl["NH4Cl"]
CultureMech_000595 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Distilled_water["Distilled water"]
CultureMech_000595 --> ing3_Distilled_water
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 2 G_PER_L | Source batch input: 2 g. Retained 2 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. | |
| 2 | Yeast extract | 2 G_PER_L | Source batch input: 2 g. Retained 2 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. | |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Source batch input: 0.5 g. Retained 0.5 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions. |
| 4 | Distilled water | 1 L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
Source batch input: 1000 ml distilled water = 1 L. This is not a volume percentage or a measured final-medium volume. |
Preparation
- After autoclaving and cooling add:
- Final pH 7.0
Solutions
Riboflavin stock
Dose: 0.005 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Riboflavin | 0.2 MG_PER_ML | Source-explicit riboflavin stock strength: 0.2 mg/ml. The source's 5 ml is a stock addition volume, not 5 g/L or 5% v/v. No final-medium concentration is inferred. |
Source batch dose: 5 ml stock / 1000 ml per L = 0.005 L. This is an input volume, not a dose per liter of measured final medium.
Glucose stock
Dose: 0.002 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | Source stock label: 50%; percentage basis unspecified. The flattened recipe quantity is withdrawn at this stock stage. No mass/volume percentage basis, density, stock solvent or final-medium concentration is inferred. |
Source batch dose: 2 ml stock / 1000 ml per L = 0.002 L. This is an input volume, not a dose per liter of measured final medium.
MgSO4 stock
Dose: 0.001 L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 | Source stock label: 20%; percentage basis unspecified. The flattened recipe quantity is withdrawn at this stock stage. No mass/volume percentage basis, density, stock solvent or final-medium concentration is inferred. |
Source batch dose: 1 ml stock / 1000 ml per L = 0.001 L. This is an input volume, not a dose per liter of measured final medium.
CaCl2 stock
Dose: 0.001 L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 | Source stock label: 10%; percentage basis unspecified. The flattened recipe quantity is withdrawn at this stock stage. No mass/volume percentage basis, density, stock solvent or final-medium concentration is inferred. |
Source batch dose: 1 ml stock / 1000 ml per L = 0.001 L. This is an input volume, not a dose per liter of measured final medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.537812Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1157 |
| 2026-03-13T06:18:56.674673Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000595 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.771447+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYEM MEDIUM" to "pyem_medium" for consistent matching |
| 2026-06-05T07:41:42.999484+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.572457+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |
| 2026-10-08T08:51:23.684976+00:00 | codex | CORRECTED_DSMZ1157_1164_STOCK_STAGES_AND_INPUTS | Restored stock boundaries, exact source doses and water input. Unspecified percentage bases/drop volume remain unresolved. Preserved prior history and snippets, chemical mappings and unrelated flags; corrected only verified stale link paths. No final-volume, growth or new variant inference. |