payne_seghal_gibbons_medium (CultureMech:000600)

Overview

External ontology

mediadive.medium:1160 (source link unavailable) — PAYNE, SEGHAL & GIBBONS MEDIUM

Applications

Microbial cultivation

pH

7.4

High-metal flag

True

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1160.pdf

Composition

flowchart LR
    CultureMech_000600["payne_seghal_gibbons_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_000600 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000600 --> ing1_Yeast_extract
    ing2_Trisodium_citrate["Trisodium citrate"]
    CultureMech_000600 --> ing2_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing2_Trisodium_citrate -.-> CHEBI_53258
    ing3_KCl["KCl"]
    CultureMech_000600 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000600 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000600 --> ing5_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing5_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000600 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_NaCl["NaCl"]
    CultureMech_000600 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Agar["Agar"]
    CultureMech_000600 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	Casamino acids	7.5 G_PER_L
2	Yeast extract	10 G_PER_L
3	Trisodium citrate C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate
4	KCl Cl.K 74.551 g/mol	2 G_PER_L	CHEBI:32588 potassium chloride
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	20 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
6	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.036 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate
7	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.00036 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate
8	NaCl Cl.Na 58.443 g/mol	250 G_PER_L	CHEBI:26710 sodium chloride
9	Agar	20 G_PER_L	CHEBI:2509 agar	(for solid medium)

Preparation

Adjust pH to 7.4. For solid medium add 20.0 g agar.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:13.542664Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1160
2026-01-28T19:48:32.502313Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:56.693078Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000600
2026-03-17T06:30:34.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.792587+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "PAYNE, SEGHAL & GIBBONS MEDIUM" to "payne_seghal_gibbons_medium" for consistent matching
2026-06-05T07:41:40.949606+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.553332+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-10-07T13:32:40.086487+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.