halomicrobium_katesii_medium

CultureMech:000605 archaea COMPLEX LIQUID
Original name: HALOMICROBIUM KATESII MEDIUM

Overview

External ontology
mediadive.medium:1164 (source link unavailable) — HALOMICROBIUM KATESII MEDIUM
Applications
  • Microbial cultivation
pH
7.2
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1164.pdf Stock stages and water inputs are explicit. Percentage bases, stock solvents and measured final volume are not supplied by these sources; no downstream dilution or growth claim is inferred. Existing chemical mappings and high_metal/other historical flags are retained but not validated by this concentration repair.

Composition

flowchart LR
    CultureMech_000605["halomicrobium_katesii_medium"]
    ing0_NaHCO3["NaHCO3"]
    CultureMech_000605 --> ing0_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing0_NaHCO3 -.-> CHEBI_32139
    ing1_NaBr["NaBr"]
    CultureMech_000605 --> ing1_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing1_NaBr -.-> CHEBI_63004
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000605 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KCl["KCl"]
    CultureMech_000605 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000605 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_000605 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Glucose["Glucose"]
    CultureMech_000605 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Proteose_peptone["Proteose peptone"]
    CultureMech_000605 --> ing7_Proteose_peptone
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_000605 --> ing8_Yeast_extract
    ing9_Distilled_water["Distilled water"]
    CultureMech_000605 --> ing9_Distilled_water

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 NaHCO3
CHO3.Na 84.006 g/mol
0.166 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Source batch input: 0.166 g. Retained 0.166 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions.
2 NaBr
Br.Na 102.894 g/mol
0.35 G_PER_L CHEBI:63004
sodium bromide
 Source batch input: 0.35 g. Retained 0.35 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions.
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.916 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Source batch input: 0.916 g. Retained 0.916 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions.
4 KCl
Cl.K 74.551 g/mol
5 G_PER_L CHEBI:32588
potassium chloride
 Source batch input: 5 g. Retained 5 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions.
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
49.83 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Source batch input: 49.83 g. Retained 49.83 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions.
6 NaCl
Cl.Na 58.443 g/mol
195 G_PER_L CHEBI:26710
sodium chloride
 Source batch input: 195 g. Retained 195 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions.
7 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
 Source batch input: 1 g. Retained 1 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions.
8 Proteose peptone 5 G_PER_L Source batch input: 5 g. Retained 5 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions.
9 Yeast extract 5 G_PER_L Source batch input: 5 g. Retained 5 g/L is the provider's nominal 1000 ml recipe rate, not a measured basal or final-medium concentration after stock additions.
10 Distilled water 1 L mediadive.compound:4 (source link unavailable)
Distilled water
 Source batch input: 1000 ml distilled water = 1 L. This is not a volume percentage or a measured final-medium volume.

Preparation

  1. Adjust to pH 7.2

Solutions

FeCl3 x 6 H2O stock

Stock composition
IngredientConcentrationNotes
FeCl3 x 6 H2O Source stock label: 0.5%; percentage basis unspecified. The flattened recipe quantity is withdrawn at this stock stage. No mass/volume percentage basis, density, stock solvent or final-medium concentration is inferred.
Source batch dose: 7 drops of FeCl3 x 6 H2O stock labelled 0.5%. Drop volume and percentage basis are unspecified. Seven drops is not 7 g/L; no numeric volume, stock strength or final-medium concentration is inferred.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.559427Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1164
2026-03-13T06:18:56.742785Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000605
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.849540+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOMICROBIUM KATESII MEDIUM" to "halomicrobium_katesii_medium" for consistent matching
2026-06-05T07:41:07.848504+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.542610+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.
2026-10-08T08:51:23.684976+00:00 codex CORRECTED_DSMZ1157_1164_STOCK_STAGES_AND_INPUTS Restored stock boundaries, exact source doses and water input. Unspecified percentage bases/drop volume remain unresolved. Preserved prior history and snippets, chemical mappings and unrelated flags; corrected only verified stale link paths. No final-volume, growth or new variant inference.