nsy_medium_for_polynucleobacter

CultureMech:000609 bacterial COMPLEX LIQUID
Original name: NSY-MEDIUM FOR POLYNUCLEOBACTER

Overview

External ontology
mediadive.medium:1167 (source link unavailable) — NSY-MEDIUM FOR POLYNUCLEOBACTER
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1167.pdf

Composition

flowchart LR
    CultureMech_000609["nsy_medium_for_polynucleobacter"]
    ing0_Nutrient_broth["Nutrient broth"]
    CultureMech_000609 --> ing0_Nutrient_broth
    ing1_Soy_peptone["Soy peptone"]
    CultureMech_000609 --> ing1_Soy_peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000609 --> ing2_Yeast_extract
    ing3_Tap_water["Tap water"]
    CultureMech_000609 --> ing3_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Tap_water -.-> CHEBI_15377
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000609 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000609 --> ing5_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing5_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing6_NaHCO3["NaHCO3"]
    CultureMech_000609 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_KCl["KCl"]
    CultureMech_000609 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000609 --> ing8_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing8_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing9_Na2_EDTA["Na2-EDTA"]
    CultureMech_000609 --> ing9_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na2_EDTA -.-> CHEBI_64734
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000609 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000609 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000609 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_H3BO3["H3BO3"]
    CultureMech_000609 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000609 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000609 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000609 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000609 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Nutrient broth 1 G_PER_L
2 Soy peptone 1 G_PER_L
3 Yeast extract 1 G_PER_L
4 Tap water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.075 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.043 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
7 NaHCO3
CHO3.Na 84.006 g/mol
0.016 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 KCl
Cl.K 74.551 g/mol
0.005 G_PER_L CHEBI:32588
potassium chloride
9 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
0.0037 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
10 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
11 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
12 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. The following inorganic solution may substitute for tap water:
  2. Adjust to pH 7.2.
  3. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.574953Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1167
2026-03-13T06:18:56.794622Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000609
2026-03-17T06:30:33.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.907607+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NSY-MEDIUM FOR POLYNUCLEOBACTER" to "nsy_medium_for_polynucleobacter" for consistent matching
2026-06-05T07:41:40.102049+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.696476+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.