Overview
- External ontology
- mediadive.medium:1174 (source link unavailable) — NITRINCOLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1174.pdf
Composition
flowchart LR
CultureMech_000616["nitrincola_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_000616 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_NH4Cl["NH4Cl"]
CultureMech_000616 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Yeast_extract["Yeast extract"]
CultureMech_000616 --> ing2_Yeast_extract
ing3_Na_acetate["Na acetate"]
CultureMech_000616 --> ing3_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Na_acetate -.-> CHEBI_32954
ing4_NaCl["NaCl"]
CultureMech_000616 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Na2B4O7["Na2B4O7"]
CultureMech_000616 --> ing5_Na2B4O7
CHEBI_38892["CHEBI:38892"]
ing5_Na2B4O7 -.-> CHEBI_38892
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.25 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 | Yeast extract | 0.1 G_PER_L | ||
| 4 |
Na acetate
C2H3O2.Na 82.034 g/mol
|
10 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
17.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Na2B4O7
H4B4O9.2Na 237.251 g/mol
|
4 G_PER_L |
CHEBI:38892
disodium tetraborate
|
Preparation
- Adjust the pH to 9.0 with 1M NaOH before autoclaving.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.589892Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1174 |
| 2026-01-28T19:48:13.521064Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:56.832691Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000616 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.957017+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NITRINCOLA MEDIUM" to "nitrincola_medium" for consistent matching |
| 2026-06-05T07:41:39.760521+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.579986+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |