alkaliflexus_medium

CultureMech:000617 bacterial COMPLEX LIQUID
Original name: ALKALIFLEXUS MEDIUM

Overview

External ontology
mediadive.medium:1175 (source link unavailable) — ALKALIFLEXUS MEDIUM
Applications
  • Microbial cultivation
pH
10.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1175.pdf

Composition

flowchart LR
    CultureMech_000617["alkaliflexus_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_000617 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000617 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000617 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Na2CO3["Na2CO3"]
    CultureMech_000617 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_NaHCO3["NaHCO3"]
    CultureMech_000617 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000617 --> ing5_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing5_Na2S_x_9_H2O -.-> CHEBI_76209
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_000617 --> ing6_Yeast_extract
    ing7_Cellobiose["Cellobiose"]
    CultureMech_000617 --> ing7_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing7_Cellobiose -.-> CHEBI_17057

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.2 G_PER_L CHEBI:31206
ammonium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.05 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 Na2CO3
CO3.2Na 105.988 g/mol
7.4 G_PER_L CHEBI:29377
sodium carbonate
5 NaHCO3
CHO3.Na 84.006 g/mol
18.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
7 Yeast extract 0.2 G_PER_L
8 Cellobiose
C12H22O11 342.297 g/mol
3 G_PER_L CHEBI:17057
cellobiose

Preparation

  1. pH 10.0
  2. Prepare the medium without Na2CO3, NaHCO3, Na2S x 9 H2O, cellulobiose or yeast extract. Boil the medium in a water bath and cool under a stream of nitrogen. Add the Na2CO3 and NaHCO3 to the cooled medium (the pH should be about 10.0) and dispense the medium into Hungate tubes or serum bottles under a stream of nitrogen. Seal the tubes under nitrogen gas and autoclave. To the cooled medium at the appropriate amounts of Na2S x 9 H2O, cellulobiose and yeast extract from sterile, anaerobic stock solutions (10% Na2S x 9 H2O, 5% cellulobiose, 10% yeast extract).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.591472Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1175
2026-03-13T06:18:56.837286Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000617
2026-03-17T06:29:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.962504+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALKALIFLEXUS MEDIUM" to "alkaliflexus_medium" for consistent matching
2026-06-05T07:40:47.835349+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.781118+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.