methylomicrobium_medium

CultureMech:000622 bacterial DEFINED LIQUID
Original name: METHYLOMICROBIUM MEDIUM

Overview

External ontology
mediadive.medium:1180 (source link unavailable) — METHYLOMICROBIUM MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 8.5, 'max': 9.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1180.pdf

Composition

flowchart LR
    CultureMech_000622["methylomicrobium_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000622 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000622 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000622 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KNO3["KNO3"]
    CultureMech_000622 --> ing3_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing3_KNO3 -.-> CHEBI_63043
    ing4_NaHCO3["NaHCO3"]
    CultureMech_000622 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Na2CO3["Na2CO3"]
    CultureMech_000622 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_EDTA["EDTA"]
    CultureMech_000622 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_000622 --> ing7_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing7_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000622 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000622 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000622 --> ing10_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000622 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_Na2MoO4["Na2MoO4"]
    CultureMech_000622 --> ing12_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing12_Na2MoO4 -.-> CHEBI_75215
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000622 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_000622 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_KH2PO4["KH2PO4"]
    CultureMech_000622 --> ing15_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing15_KH2PO4 -.-> CHEBI_63036
    ing16_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_000622 --> ing16_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing16_Na2HPO4_x_12_H2O -.-> CHEBI_34683

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
5 NaHCO3
CHO3.Na 84.006 g/mol
4.20035 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 Na2CO3
CO3.2Na 105.988 g/mol
0.52994 G_PER_L CHEBI:29377
sodium carbonate
7 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.1 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 Na2MoO4
MoO4.2Na 205.916 g/mol
0.03 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
16 KH2PO4
H2O4P.K 136.084 g/mol
14 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
17 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
30 G_PER_L CHEBI:34683
disodium hydrogenphosphate

Preparation

  1. Final pH of the medium: 8.5-9.0
  2. Dispense the medium into the culture vessels. For small volumes serum bottles or Hungate tubes may be used. The gas phase to liquid ratio should be about 10:1. Use a gas phase containing air, 50% methane and autoclave at 121°C for 15 minutes. When the growth medium is cool the NaHCO3, the Na2CO3 and phosphate buffer are added.
  3. Liquid cultures should be grown with shaking.
  4. To the sterile medium add from sterile stock solutions:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.601112Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1180
2026-03-13T06:18:56.866952Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000622
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.996375+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOMICROBIUM MEDIUM" to "methylomicrobium_medium" for consistent matching
2026-05-20T06:01:56.826549+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '8.5-9' -> {'min': 8.5, 'max': 9.0}
2026-06-05T07:41:33.191117+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.535837+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.582209+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.