methylocella_silverstris_medium

CultureMech:000623 bacterial DEFINED LIQUID
Original name: METHYLOCELLA SILVERSTRIS MEDIUM

Overview

External ontology
mediadive.medium:1181 (source link unavailable) — METHYLOCELLA SILVERSTRIS MEDIUM
Applications
  • Microbial cultivation
pH
5.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1181.pdf

Composition

flowchart LR
    CultureMech_000623["methylocella_silverstris_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000623 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_000623 --> ing1_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing1_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing2_Fe_III_NH4_EDTA["Fe(III)NH4-EDTA"]
    CultureMech_000623 --> ing2_Fe_III_NH4_EDTA
    ing3_NaNO3["NaNO3"]
    CultureMech_000623 --> ing3_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing3_NaNO3 -.-> CHEBI_63005
    ing4_KH2PO4["KH2PO4"]
    CultureMech_000623 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_000623 --> ing5_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing5_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000623 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000623 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_H3BO3["H3BO3"]
    CultureMech_000623 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_CuCl2_x_6_H2O["CuCl2 x 6 H2O"]
    CultureMech_000623 --> ing9_CuCl2_x_6_H2O
    CHEBI_49553["CHEBI:49553"]
    ing9_CuCl2_x_6_H2O -.-> CHEBI_49553
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000623 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000623 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_000623 --> ing12_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing12_NaH2PO4_x_2_H2O -.-> CHEBI_37585

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
0.006 G_PER_L CHEBI:91243
calcium chloride hexahydrate
3 Fe(III)NH4-EDTA 0.004 G_PER_L
4 NaNO3
NO3.Na 84.994 g/mol
0.2 G_PER_L CHEBI:63005
sodium nitrate
5 KH2PO4
H2O4P.K 136.084 g/mol
0.272 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
6.447 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 [Merged 2 duplicates: 0.717, 5.73]
7 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.01 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.003 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
10 CuCl2 x 6 H2O
Cl2Cu 134.452 g/mol
0.001 G_PER_L CHEBI:49553
copper(II) chloride
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
28.71 G_PER_L CHEBI:37585
sodium dihydrogenphosphate

Preparation

  1. Dispense the medium into the culture vessels. For small volumes serum bottles or Hungate tubes may be used. The gas phase to liquid ratio should be about 1:10. Use a gas phase containing air, 20-30% methane and autoclave at 121°C for 15 minutes. When the growth medium is cool the phosphate buffer is added.
  2. Autoclave at 121°C for 15 min.
  3. Add to the sterile medium from a filter sterilised phosphate buffer:
  4. Liquid cultures should be grown with shaking.
  5. Final pH of the medium is 5.8.
  6. pH 5.8

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.604051Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1181
2026-03-13T03:09:13.532478Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:56.875440Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000623
2026-03-17T06:30:20.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.005999+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOCELLA SILVERSTRIS MEDIUM" to "methylocella_silverstris_medium" for consistent matching
2026-06-05T07:41:33.092858+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.544871+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.