Overview
- External ontology
- mediadive.medium:1185 (source link unavailable) — SMB MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.1}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1185.pdf
Composition
flowchart LR
CultureMech_000627["smb_medium"]
ing0_NaCl["NaCl"]
CultureMech_000627 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000627 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000627 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4NO3["NH4NO3"]
CultureMech_000627 --> ing3_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing3_NH4NO3 -.-> CHEBI_63038
ing4_KH2PO4["KH2PO4"]
CultureMech_000627 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Na2HPO4["Na2HPO4"]
CultureMech_000627 --> ing5_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4 -.-> CHEBI_34683
ing6_Na2CO3["Na2CO3"]
CultureMech_000627 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
ing7_MnSO4_x_2_H2O["MnSO4 x 2 H2O"]
CultureMech_000627 --> ing7_MnSO4_x_2_H2O
CHEBI_86356["CHEBI:86356"]
ing7_MnSO4_x_2_H2O -.-> CHEBI_86356
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000627 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_Tryptone["Tryptone"]
CultureMech_000627 --> ing9_Tryptone
ing10_Yeast_extract["Yeast extract"]
CultureMech_000627 --> ing10_Yeast_extract
ing11_Agar["Agar"]
CultureMech_000627 --> ing11_Agar
CHEBI_2509["CHEBI:2509"]
ing11_Agar -.-> CHEBI_2509
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
50 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NH4NO3
H4N.NO3 80.043 g/mol
|
1 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
3 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.1 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 8 |
MnSO4 x 2 H2O
2H2O.Mn.O4S 187.031 g/mol
|
0.02 G_PER_L |
CHEBI:86356
manganese(II) sulfate dihydrate
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 10 | Tryptone | 5 G_PER_L | ||
| 11 | Yeast extract | 2 G_PER_L | ||
| 12 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- pH 6.8 - 7.1
- The medium may be solidified by adding 15.0 g/l agar.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.617553Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1185 |
| 2026-01-28T19:48:18.053238Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:56.909196Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000627 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.048184+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SMB MEDIUM" to "smb_medium" for consistent matching |
| 2026-05-20T06:02:05.298752+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7.1' -> {'min': 6.8, 'max': 7.1} |
| 2026-06-05T07:41:45.929138+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.521844+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |