dap_nutrient_agar

CultureMech:000633 bacterial COMPLEX SOLID_AGAR
Original name: DAP-NUTRIENT AGAR

Overview

External ontology
mediadive.medium:118 (source link unavailable) — DAP-NUTRIENT AGAR
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium118.pdf

Composition

flowchart LR
    CultureMech_000633["dap_nutrient_agar"]
    ing0_Peptone["Peptone"]
    CultureMech_000633 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_000633 --> ing1_Meat_extract
    ing2_Diaminopimelic_acid["Diaminopimelic acid"]
    CultureMech_000633 --> ing2_Diaminopimelic_acid
    CHEBI_23673["CHEBI:23673"]
    ing2_Diaminopimelic_acid -.-> CHEBI_23673
    ing3_Agar["Agar"]
    CultureMech_000633 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L
2 Meat extract 3 G_PER_L
3 Diaminopimelic acid
C7H14N2O4 190.199 g/mol
0.1 G_PER_L CHEBI:23673
2,6-diaminopimelic acid
4 Agar 15 G_PER_L CHEBI:2509
agar
(if necessary)

Preparation

  1. Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-26T17:53:10.203419Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 118
2026-03-13T06:18:56.953429Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000633
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.099626+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DAP-NUTRIENT AGAR" to "dap_nutrient_agar" for consistent matching
2026-06-05T07:40:55.289187+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.