Overview
- External ontology
- mediadive.medium:1191 (source link unavailable) — EGGC
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1191.pdf
Composition
flowchart LR
CultureMech_000634["eggc"]
ing0_Na_acetate["Na-acetate"]
CultureMech_000634 --> ing0_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing0_Na_acetate -.-> CHEBI_32954
ing1_Casamino_acids["Casamino acids"]
CultureMech_000634 --> ing1_Casamino_acids
ing2_Glucose["Glucose"]
CultureMech_000634 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Agar["Agar"]
CultureMech_000634 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Demineralized_water["Demineralized water"]
CultureMech_000634 --> ing4_Demineralized_water
CHEBI_15377["CHEBI:15377"]
ing4_Demineralized_water -.-> CHEBI_15377
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_000634 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_KCl["KCl"]
CultureMech_000634 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000634 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000634 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_CaCO3["CaCO3"]
CultureMech_000634 --> ing9_CaCO3
CHEBI_3311["CHEBI:3311"]
ing9_CaCO3 -.-> CHEBI_3311
ing10_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000634 --> ing10_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing10_FeCl3_x_6_H2O -.-> CHEBI_86254
ing11_Na2HPO4["Na2HPO4"]
CultureMech_000634 --> ing11_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing11_Na2HPO4 -.-> CHEBI_34683
ing12_NaH2PO4["NaH2PO4"]
CultureMech_000634 --> ing12_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing12_NaH2PO4 -.-> CHEBI_37585
ing13_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000634 --> ing13_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing13_Calcium_pantothenate -.-> CHEBI_31345
ing14_Biotin["Biotin"]
CultureMech_000634 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Folic_acid["Folic acid"]
CultureMech_000634 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_000634 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.3 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 2 | Casamino acids | 0.3 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.6 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 5 |
Demineralized water
H2O 18.015 g/mol
|
2120.0 G_PER_L |
CHEBI:15377
water
|
[Merged 4 duplicates: 1000.0, 1000.0, 20.0, 100.0] |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
CaCO3
CO3.Ca 100.086 g/mol
|
0.2 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 11 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.005 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 12 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
124 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 13 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
15.4 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 14 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.001 G_PER_L |
CHEBI:15956
biotin
|
|
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 17 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Please add after autoclaving:
- Adjust pH to 7.2
Solutions
Vitamin solution
Dose: 5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Riboflavin | 0.1 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.5 G_PER_L | |
| Nicotinic acid | 0.5 G_PER_L | |
| Pyridoxine hydrochloride | 0.5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.635241Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1191 |
| 2026-01-28T19:48:28.513759Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:17.883315Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:18:56.961155Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000634 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.108657+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "EGGC" to "eggc" for consistent matching |
| 2026-06-05T07:40:59.422315+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:38.029227+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:56.656568+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T07:05:22.172563+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Vitamin solution' @ 5 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |