cultivation_medium_for_chlamydiae (CultureMech:000636)

Overview

External ontology

mediadive.medium:1193 (source link unavailable) — CULTIVATION MEDIUM FOR CHLAMYDIAE

Applications

Microbial cultivation

Notes

DSMZ Medium 1193 prepares a 50.5 ml L929/HeLa cell-culture medium from 45.0 ml IMDM, 5.0 ml fetal bovine serum, and 0.5 ml 100x amino-acid stock, then filter-sterilizes it before Chlamydiae cultivation at 37 C plus 5% CO2.

Composition

flowchart LR
    CultureMech_000636["cultivation_medium_for_chlamydiae"]
    ing0_IMDM_Medium["IMDM-Medium"]
    CultureMech_000636 --> ing0_IMDM_Medium
    ing1_Fetal_bovine_serum["Fetal bovine serum"]
    CultureMech_000636 --> ing1_Fetal_bovine_serum
    ing2_Aminoacids__100_x_["Aminoacids (100 x)"]
    CultureMech_000636 --> ing2_Aminoacids__100_x_

Ingredients (3)

#	Ingredient	Concentration	Ontology	Notes
1	IMDM-Medium	891.089109 ML_PER_L	mediadive.compound:1069 (source link unavailable) IMDM-Medium	DSMZ Medium 1193 lists 45.0 ml IMDM-Medium in a 50.5 ml L929/HeLa cell-culture medium batch.
2	Fetal bovine serum	99.009901 ML_PER_L	mediadive.compound:954 (source link unavailable) Fetal bovine serum	DSMZ Medium 1193 lists 5.0 ml fetal bovine serum in a 50.5 ml L929/HeLa cell-culture medium batch.
3	Aminoacids (100 x)	9.90099 ML_PER_L	mediadive.compound:1070 (source link unavailable) Aminoacids	DSMZ Medium 1193 lists 0.5 ml 100x amino-acid stock in a 50.5 ml L929/HeLa cell-culture medium batch.

Preparation

Combine 45.0 ml IMDM-Medium, 5.0 ml fetal bovine serum, and 0.5 ml 100x amino acids.
Filter sterilize through a 0.2 um membrane.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:13.639960Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1193
2026-03-13T06:18:56.969991Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000636
2026-03-17T19:45:08.119495+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "CULTIVATION MEDIUM FOR CHLAMYDIAE" to "cultivation_medium_for_chlamydiae" for consistent matching
2026-09-09T00:00:00-07:00	repair_dsmz_host_cell_score25.py	RESOLVED_DSMZ_HOST_CELL_SCORE25_GRAPH	DSMZ Medium 1193 prepares a 50.5 ml L929/HeLa cell-culture medium from 45.0 ml IMDM, 5.0 ml fetal bovine serum, and 0.5 ml 100x amino-acid stock, then filter-sterilizes it before Chlamydiae cultivation at 37 C plus 5% CO2.