Overview
- External ontology
- mediadive.medium:1194 (source link unavailable) — HALORUBRUM CALIFORNIENSE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1194.pdf
Composition
flowchart LR
CultureMech_000637["halorubrum_californiense_medium"]
ing0_NaCl["NaCl"]
CultureMech_000637 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000637 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000637 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_000637 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaBr["NaBr"]
CultureMech_000637 --> ing4_NaBr
CHEBI_63004["CHEBI:63004"]
ing4_NaBr -.-> CHEBI_63004
ing5_NaHCO3["NaHCO3"]
CultureMech_000637 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000637 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Yeast_extract["Yeast extract"]
CultureMech_000637 --> ing7_Yeast_extract
ing8_Agar["Agar"]
CultureMech_000637 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
156 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
26 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
40.6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
4 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaBr
Br.Na 102.894 g/mol
|
0.46 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.12 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.66 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 | Yeast extract | 10 G_PER_L | ||
| 9 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust to pH 7.2 and autoclave.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.641536Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1194 |
| 2026-03-13T06:18:56.974794Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000637 |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.124731+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALORUBRUM CALIFORNIENSE MEDIUM" to "halorubrum_californiense_medium" for consistent matching |
| 2026-06-05T07:41:08.162674+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.650097+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |