Overview
- External ontology
- mediadive.medium:1200 (source link unavailable) — ANAEROMYXOBACTER-MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1200.pdf
Composition
flowchart LR
CultureMech_000650["anaeromyxobacter_medium"]
ing0_NaCl["NaCl"]
CultureMech_000650 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000650 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KH2PO4["KH2PO4"]
CultureMech_000650 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_000650 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KCl["KCl"]
CultureMech_000650 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000650 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Na_acetate["Na-acetate"]
CultureMech_000650 --> ing6_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Na_acetate -.-> CHEBI_32954
ing7_Resazurin["Resazurin"]
CultureMech_000650 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_NaHCO3["NaHCO3"]
CultureMech_000650 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_DL_Dithiothreitol["DL-Dithiothreitol"]
CultureMech_000650 --> ing9_DL_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing9_DL_Dithiothreitol -.-> CHEBI_18320
ing10_L_Cysteine["L-Cysteine"]
CultureMech_000650 --> ing10_L_Cysteine
CHEBI_17561["CHEBI:17561"]
ing10_L_Cysteine -.-> CHEBI_17561
ing11_Na2S_x_H2O["Na2S x H2O"]
CultureMech_000650 --> ing11_Na2S_x_H2O
CHEBI_76208["CHEBI:76208"]
ing11_Na2S_x_H2O -.-> CHEBI_76208
ing12_Disodium_fumarate["Disodium fumarate"]
CultureMech_000650 --> ing12_Disodium_fumarate
CHEBI_115156["CHEBI:115156"]
ing12_Disodium_fumarate -.-> CHEBI_115156
ing13_Biotin["Biotin"]
CultureMech_000650 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
ing14_Folic_acid["Folic acid"]
CultureMech_000650 --> ing14_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing14_Folic_acid -.-> CHEBI_27470
ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000650 --> ing15_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing16_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000650 --> ing16_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing16_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing17_Riboflavin["Riboflavin"]
CultureMech_000650 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_000650 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_000650 --> ing19_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing19_D_Calcium_pantothenate -.-> CHEBI_31345
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_000650 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000650 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Lipoic_acid["Lipoic acid"]
CultureMech_000650 --> ing22_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing22_Lipoic_acid -.-> CHEBI_16494
ing23_HCl["HCl"]
CultureMech_000650 --> ing23_HCl
CHEBI_17883["CHEBI:17883"]
ing23_HCl -.-> CHEBI_17883
ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000650 --> ing24_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
ing25_ZnCl2["ZnCl2"]
CultureMech_000650 --> ing25_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing25_ZnCl2 -.-> CHEBI_49976
ing26_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000650 --> ing26_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing26_MnCl2_x_4_H2O -.-> CHEBI_86368
ing27_H3BO3["H3BO3"]
CultureMech_000650 --> ing27_H3BO3
CHEBI_33118["CHEBI:33118"]
ing27_H3BO3 -.-> CHEBI_33118
ing28_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000650 --> ing28_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing28_CoCl2_x_6_H2O -.-> CHEBI_53503
ing29_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000650 --> ing29_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing29_CuCl2_x_2_H2O -.-> CHEBI_86318
CultureMech_000650 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
1.10619 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.553097 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.221239 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.331858 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.331858 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0165929 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.442478 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
50 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
DL-Dithiothreitol
C4H10O2S2 154.256 g/mol
|
7.7 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
|
| 11 |
L-Cysteine
C3H7NO2S 121.161 g/mol
|
0.75 G_PER_L |
CHEBI:17561
L-cysteine
|
|
| 12 |
Na2S x H2O
2Na.S 78.047 g/mol
|
0.8 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
|
| 13 |
Disodium fumarate
C4H2O4.2Na 160.036 g/mol
|
80 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 15 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 16 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 17 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 20 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 21 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 24 |
HCl
HCl 36.461 g/mol
|
1.925 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 25 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.4881 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 26 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0694444 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 27 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0992064 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 28 |
H3BO3
H3BO3 61.833 g/mol
|
0.297619 G_PER_L |
CHEBI:33118
boric acid
|
|
| 29 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.188492 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 30 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.00198413 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 31 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0238095 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 32 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0357143 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 33 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 34 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 35 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Prepare solutions A, B, and D separately and anaerobically under 80% N2 + 20% CO2 and autoclave. Filter-sterilize solutions C, E and F and outgas with N2. After cooling, add about 1 ml solution B per 10 ml solution A, by this way adjusting pH to 7.2. Add 0.2 ml solution C, 0.2 ml solution D, 0.2 ml solution F and 0.05 ml solution E, each per 10 ml solution A.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.662367Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1200 |
| 2026-01-28T19:48:36.930042Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:57.090042Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000650 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.251890+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANAEROMYXOBACTER-MEDIUM" to "anaeromyxobacter_medium" for consistent matching |
| 2026-06-05T07:40:49.145049+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.327859+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |