Overview
- External ontology
- mediadive.medium:1201 (source link unavailable) — ALICYCLOBACILLUS FERROOXYDANS MEDIUM
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1201.pdf
Composition
flowchart LR
CultureMech_000651["alicyclobacillus_ferrooxydans_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000651 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_000651 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_K2HPO4["K2HPO4"]
CultureMech_000651 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_K2S4O6["K2S4O6"]
CultureMech_000651 --> ing3_K2S4O6
CHEBI_86466["CHEBI:86466"]
ing3_K2S4O6 -.-> CHEBI_86466
ing4_KCl["KCl"]
CultureMech_000651 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000651 --> ing5_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing5_MnSO4_x_H2O -.-> CHEBI_86364
ing6_Yeast_extract["Yeast extract"]
CultureMech_000651 --> ing6_Yeast_extract
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000651 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.537634 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.430108 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.215054 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
K2S4O6
2K.O6S4 302.458 g/mol
|
0.16129 G_PER_L |
CHEBI:86466
potassium tetrathionate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.107527 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.0107527 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 7 | Yeast extract | 0.215054 G_PER_L | ||
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
200 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Preparation
- Autoclave solution A, cool down to 50°C and combine solution A with 70 ml of solution B.
- Adjust pH to 1.8 - 2.5 with H2SO4.
- Dissolve in 0.2 N H2SO4 and fill up to a volume of 100 ml. Filter sterilize.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.668512Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1201 |
| 2026-01-28T19:48:29.905689Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:57.098724Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000651 |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.261810+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALICYCLOBACILLUS FERROOXYDANS MEDIUM" to "alicyclobacillus_ferrooxydans_medium" for consistent matching |
| 2026-06-05T07:40:47.764831+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.754857+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |