Overview
- External ontology
- mediadive.medium:1209 (source link unavailable) — ALICYCOBACILLUS POHLIAE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1209.pdf
Composition
flowchart LR
CultureMech_000659["alicycobacillus_pohliae_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_000659 --> ing0_Tryptone
ing1_D_Glucose["D-Glucose"]
CultureMech_000659 --> ing1_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing1_D_Glucose -.-> CHEBI_17634
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_000659 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000659 --> ing3_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
ing4_Agar["Agar"]
CultureMech_000659 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 5 G_PER_L | ||
| 2 |
D-Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.07 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if required) |
Preparation
- Adjust pH to 5.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.701634Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1209 |
| 2026-03-13T06:18:57.162543Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000659 |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.387764+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALICYCOBACILLUS POHLIAE MEDIUM" to "alicycobacillus_pohliae_medium" for consistent matching |
| 2026-06-05T07:40:47.779609+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.761158+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |