Overview
- External ontology
- mediadive.medium:1216 (source link unavailable) — SALINISPHAERA HYDROTHERMALIS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1216.pdf
Composition
flowchart LR
CultureMech_000674["salinisphaera_hydrothermalis_medium"]
ing0_NaCl["NaCl"]
CultureMech_000674 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_000674 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000674 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_Bacto_peptone["Bacto peptone"]
CultureMech_000674 --> ing3_Bacto_peptone
ing4_Yeast_extract["Yeast extract"]
CultureMech_000674 --> ing4_Yeast_extract
ing5_Agar["Agar"]
CultureMech_000674 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
24 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.7 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
7 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 | Bacto peptone | 2.5 G_PER_L | ||
| 5 | Yeast extract | 3 G_PER_L | ||
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Add the vitamin B12 solution from a filter sterilised stock solution to the autoclaved, cooled medium. Add 15.0 g/l agar for solid media.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.739326Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1216 |
| 2026-03-13T06:18:57.344395Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000674 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.605353+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SALINISPHAERA HYDROTHERMALIS MEDIUM" to "salinisphaera_hydrothermalis_medium" for consistent matching |
| 2026-06-05T07:41:45.076414+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |