Overview
- External ontology
- mediadive.medium:1218 (source link unavailable) — ROSEINATRONOBACTER MONICUS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 9.0, 'max': 9.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1218.pdf
Composition
flowchart LR
CultureMech_000676["roseinatronobacter_monicus_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_000676 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_000676 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000676 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_Na2SO4["Na2SO4"]
CultureMech_000676 --> ing3_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing3_Na2SO4 -.-> CHEBI_32149
ing4_NaNO3["NaNO3"]
CultureMech_000676 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_NaCl["NaCl"]
CultureMech_000676 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_KCl["KCl"]
CultureMech_000676 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_NaHCO3["NaHCO3"]
CultureMech_000676 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Na2CO3["Na2CO3"]
CultureMech_000676 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_000676 --> ing9_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing9_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing10_Vitamin_B12["Vitamin B12"]
CultureMech_000676 --> ing10_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamin_B12 -.-> CHEBI_176843
ing11_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_000676 --> ing11_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing11_Sodium_pyruvate -.-> CHEBI_50144
ing12_Sodium_acetate["Sodium acetate"]
CultureMech_000676 --> ing12_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing12_Sodium_acetate -.-> CHEBI_32954
ing13_Peptone["Peptone"]
CultureMech_000676 --> ing13_Peptone
ing14_Yeast_extract["Yeast extract"]
CultureMech_000676 --> ing14_Yeast_extract
ing15_HCl["HCl"]
CultureMech_000676 --> ing15_HCl
CHEBI_17883["CHEBI:17883"]
ing15_HCl -.-> CHEBI_17883
ing16_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000676 --> ing16_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing16_FeCl2_x_4_H2O -.-> CHEBI_86249
ing17_ZnCl2["ZnCl2"]
CultureMech_000676 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000676 --> ing18_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
ing19_H3BO3["H3BO3"]
CultureMech_000676 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000676 --> ing20_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000676 --> ing21_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000676 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000676 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.5 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
0.4 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
40 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 11 | Vitamin B12 | 1e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 12 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 13 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 14 | Peptone | 1 G_PER_L | ||
| 15 | Yeast extract | 1 G_PER_L | ||
| 16 |
HCl
HCl 36.461 g/mol
|
5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 17 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.49254 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0696517 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 19 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.199005 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.00597015 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.189055 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 22 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.00199005 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0238806 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0358209 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Autoclave the NaHCO3 and Na2CO3 separately and add to the medium. Add the vitamin B12 solution from a filter sterilised stock solution to the autoclaved, cooled medium. Final is pH 9.0 - 9.5
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.744884Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1218 |
| 2026-03-13T06:18:57.362931Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000676 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.627169+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ROSEINATRONOBACTER MONICUS MEDIUM" to "roseinatronobacter_monicus_medium" for consistent matching |
| 2026-05-20T06:02:04.328921+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '9-9.5' -> {'min': 9.0, 'max': 9.5} |
| 2026-06-05T07:41:44.621673+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.065631+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |