Overview
- External ontology
- mediadive.medium:1219 (source link unavailable) — METHYLOVIRGULA LIGNI MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 5.0, 'max': 5.8}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1219.pdf
Composition
flowchart LR
CultureMech_000677["methylovirgula_ligni_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000677 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_000677 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000677 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000677 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Yeast_extract["Yeast extract"]
CultureMech_000677 --> ing4_Yeast_extract
ing5_Methanol["Methanol"]
CultureMech_000677 --> ing5_Methanol
CHEBI_17790["CHEBI:17790"]
ing5_Methanol -.-> CHEBI_17790
ing6_Agar["Agar"]
CultureMech_000677 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_EDTA["EDTA"]
CultureMech_000677 --> ing7_EDTA
CHEBI_4735["CHEBI:4735"]
ing7_EDTA -.-> CHEBI_4735
ing8_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
CultureMech_000677 --> ing8_CuCl2_x_5_H2O
CHEBI_91245["CHEBI:91245"]
ing8_CuCl2_x_5_H2O -.-> CHEBI_91245
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000677 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000677 --> ing10_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000677 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000677 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_Na2MoO4["Na2MoO4"]
CultureMech_000677 --> ing13_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing13_Na2MoO4 -.-> CHEBI_75215
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Yeast extract | 0.1 G_PER_L | ||
| 6 |
Methanol
CH4O 32.042 g/mol
|
0.792 G_PER_L |
CHEBI:17790
methanol
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 8 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 9 |
CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
|
0.1 G_PER_L |
CHEBI:91245
copper(II) chloride pentahydrate
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
0.03 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Preparation
- Adjust to pH 5.0 - 5.8. Prepare the medium in flasks or tubes with screw caps. The methanol may either be filter sterilised or autoclaved in half full serum bottles sealed with a rubber stopper and a crimp closure. Add to the cooled autoclaved medium. Agar may be added at 15 g/l for solid media.
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- METHYLOVIRGULA LIGNI
- Term
- Id
- NCBITaxon:569860
- Label
- Methylovirgula ligni
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.748810Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1219 |
| 2026-02-20T07:49:55.000000Z | organism-data-backfill | Added curated organism data | Added organism: METHYLOVIRGULA LIGNI, culture_type: isolate |
| 2026-03-13T06:18:57.371490Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000677 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.637635+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOVIRGULA LIGNI MEDIUM" to "methylovirgula_ligni_medium" for consistent matching |
| 2026-05-20T06:01:57.079770+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5-5.8' -> {'min': 5.0, 'max': 5.8} |
| 2026-06-05T07:41:33.550296+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.721910+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |