medium_for_sulfospirillum_dsm_806

CultureMech:000678 bacterial COMPLEX LIQUID
Original name: MEDIUM FOR SULFOSPIRILLUM DSM 806

Overview

External ontology
mediadive.medium:121 (source link unavailable) — MEDIUM FOR SULFOSPIRILLUM DSM 806
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium121.pdf

Composition

flowchart LR
    CultureMech_000678["medium_for_sulfospirillum_dsm_806"]
    ing0_Na_aspartate["Na-aspartate"]
    CultureMech_000678 --> ing0_Na_aspartate
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000678 --> ing1_Yeast_extract
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000678 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000678 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_K2HPO4["K2HPO4"]
    CultureMech_000678 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_NaH2PO4["NaH2PO4"]
    CultureMech_000678 --> ing5_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing5_NaH2PO4 -.-> CHEBI_37585
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000678 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Resazurin["Resazurin"]
    CultureMech_000678 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_HCl["HCl"]
    CultureMech_000678 --> ing8_HCl
    CHEBI_17883["CHEBI:17883"]
    ing8_HCl -.-> CHEBI_17883
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000678 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_ZnCl2["ZnCl2"]
    CultureMech_000678 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000678 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_000678 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000678 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000678 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000678 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000678 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Na-aspartate 10 G_PER_L
2 Yeast extract 0.2 G_PER_L
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.028 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 K2HPO4
HO4P.2K 174.174 g/mol
0.75 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 NaH2PO4
H2O4P.Na 119.976 g/mol
0.25 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
7 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.25 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
8 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
9 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
10 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
11 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 7.0. Phosphate and cysteine should be dissolved together in 1/10 vol. of water and sterilized separately.
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.246875Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 121
2026-03-13T06:18:57.379650Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000678
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.647027+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR SULFOSPIRILLUM DSM 806" to "medium_for_sulfospirillum_dsm_806" for consistent matching
2026-06-05T07:41:30.000303+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.407960+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.