rhodovulum_marinum_medium

CultureMech:000681 bacterial DEFINED LIQUID
Original name: RHODOVULUM MARINUM MEDIUM

Overview

External ontology
mediadive.medium:1222 (source link unavailable) — RHODOVULUM MARINUM MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1222.pdf

Composition

flowchart LR
    CultureMech_000681["rhodovulum_marinum_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000681 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NaCl["NaCl"]
    CultureMech_000681 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000681 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000681 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000681 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Sorbitol["Sorbitol"]
    CultureMech_000681 --> ing5_Sorbitol
    CHEBI_30911["CHEBI:30911"]
    ing5_Sorbitol -.-> CHEBI_30911
    ing6_Vitamin_B12["Vitamin B12"]
    CultureMech_000681 --> ing6_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing6_Vitamin_B12 -.-> CHEBI_176843
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_000681 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000681 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000681 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
    CultureMech_000681 --> ing10_MnCl2_x_2_H2O
    CHEBI_131395["CHEBI:131395"]
    ing10_MnCl2_x_2_H2O -.-> CHEBI_131395
    ing11_ZnCl2["ZnCl2"]
    CultureMech_000681 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000681 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000681 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_H3BO3["H3BO3"]
    CultureMech_000681 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000681 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
3 NH4Cl
Cl.H4N 53.492 g/mol
0.6 G_PER_L CHEBI:31206
ammonium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 Sorbitol
C6H14O6 182.172 g/mol
3 G_PER_L CHEBI:30911
glucitol
7 Vitamin B12 1 G_PER_L CHEBI:176843
vitamin B12
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
5.2 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
9 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
10 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
11 MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
0.1 G_PER_L CHEBI:131395
manganese(II) chloride dihydrate
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 H3BO3
H3BO3 61.833 g/mol
0.062 G_PER_L CHEBI:33118
boric acid
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.017 G_PER_L CHEBI:86318
copper(II) chloride dihydrate

Preparation

  1. Prepare the medium under a nitrogen atmosphere, dispense into tubes or bottles fitted with rubber stoppers so that they are half full. Add the vitamin B12 from a filter-sterilised stock solution. Either sorbitol or sodium pyruvate may serve as the carbon source. The final pH of the medium should be 6.8.
  2. Adjust pH to 6.8.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.756696Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1222
2026-03-13T06:18:57.399271Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000681
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.671040+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RHODOVULUM MARINUM MEDIUM" to "rhodovulum_marinum_medium" for consistent matching
2026-06-05T07:41:44.496557+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.022046+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.