ectothiorhodospira_variabilis_medium

Overview

External ontology

mediadive.medium:1224 (source link unavailable) — ECTOTHIORHODOSPIRA VARIABILIS MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 9.0, 'max': 9.5}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1224.pdf

Composition

flowchart LR
    CultureMech_000683["ectothiorhodospira_variabilis_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000683 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2CO3["Na2CO3"]
    CultureMech_000683 --> ing1_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing1_Na2CO3 -.-> CHEBI_29377
    ing2_NaHCO3["NaHCO3"]
    CultureMech_000683 --> ing2_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing2_NaHCO3 -.-> CHEBI_32139
    ing3_KCl["KCl"]
    CultureMech_000683 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_K2HPO4["K2HPO4"]
    CultureMech_000683 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000683 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_NH4Cl["NH4Cl"]
    CultureMech_000683 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000683 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000683 --> ing8_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_000683 --> ing9_Yeast_extract
    ing10_Na_acetate["Na acetate"]
    CultureMech_000683 --> ing10_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing10_Na_acetate -.-> CHEBI_32954
    ing11_Vitamin_B12["Vitamin B12"]
    CultureMech_000683 --> ing11_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing11_Vitamin_B12 -.-> CHEBI_176843
    ing12_HCl["HCl"]
    CultureMech_000683 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000683 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_ZnCl2["ZnCl2"]
    CultureMech_000683 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000683 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_H3BO3["H3BO3"]
    CultureMech_000683 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000683 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000683 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000683 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000683 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

Preparation

1. Final pH 9.0 - 9.5
2. Prepare and boil the medium in the absence of Na2S x 9 H2O, NaHCO3, Vitamin B12, CaCl2 x 2 H2O, MgCl2 x 6 H2O. Cool under a stream of nitrogen and add the remaining components, except vitamin B12 and Na2S2O3 x 5 H2O. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. Add the vitamin B12 (filter sterilised) and Na2S2O3 x 5 H2O from sterile stock solutions.
3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance