Overview
- External ontology
- mediadive.medium:122 (source link unavailable) — ACETIVIBRIO MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium122.pdf
Composition
flowchart LR
CultureMech_000688["acetivibrio_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_000688 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000688 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KH2PO4["KH2PO4"]
CultureMech_000688 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4["K2HPO4"]
CultureMech_000688 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000688 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Na2___glycerophosphate_x_5_H2O["Na2-ß-glycerophosphate x 5 H2O"]
CultureMech_000688 --> ing5_Na2___glycerophosphate_x_5_H2O
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000688 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_L_Glutathione["L-Glutathione"]
CultureMech_000688 --> ing7_L_Glutathione
ing8_Yeast_extract["Yeast extract"]
CultureMech_000688 --> ing8_Yeast_extract
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_000688 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_Cellobiose["Cellobiose"]
CultureMech_000688 --> ing10_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing10_Cellobiose -.-> CHEBI_17057
ing11_Cellulose["Cellulose"]
CultureMech_000688 --> ing11_Cellulose
CHEBI_18246["CHEBI:18246"]
ing11_Cellulose -.-> CHEBI_18246
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.6 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.43 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.13 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Na2-ß-glycerophosphate x 5 H2O | 6 G_PER_L | ||
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0011 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 | L-Glutathione | 0.25 G_PER_L | ||
| 9 | Yeast extract | 4.5 G_PER_L | ||
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Cellobiose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 12 |
Cellulose
(C6H10O5)n.H2O 180.156 g/mol
|
10 G_PER_L |
CHEBI:18246
(1->4)-beta-D-glucan
|
Optional ingredient |
Preparation
- Dissolve ingredients except cellobiose, sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Then adjust pH to 7.0 - 7.2, distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Cellobiose is added to the sterile medium from an anoxic 10% (w/v) stock solution prepared under 100% N2 gas and sterilized by filtration. Some strains can be adapted to cellulose as substrate using 10.00 g/l cellulose (Avicel or MN 301, MACHEREY-NAGEL).
- Note: A white precipitate forms after mixing the ingredients of this medium, but this has no negative effect on growth.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.249982Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 122 |
| 2026-03-13T06:18:57.464862Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000688 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.747576+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACETIVIBRIO MEDIUM" to "acetivibrio_medium" for consistent matching |
| 2026-05-20T06:01:07.052242+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:40:46.697272+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.375910+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |