Overview
- External ontology
- mediadive.medium:1231 (source link unavailable) — HALOPLASMA CONTRACTILE MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.5, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1231.pdf
Composition
flowchart LR
CultureMech_000690["haloplasma_contractile_medium"]
ing0_NaCl["NaCl"]
CultureMech_000690 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KH2PO4["KH2PO4"]
CultureMech_000690 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Yeast_extract["Yeast extract"]
CultureMech_000690 --> ing2_Yeast_extract
ing3_Peptone["Peptone"]
CultureMech_000690 --> ing3_Peptone
ing4_Starch["Starch"]
CultureMech_000690 --> ing4_Starch
CHEBI_28017["CHEBI:28017"]
ing4_Starch -.-> CHEBI_28017
ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000690 --> ing5_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_x_9_H2O -.-> CHEBI_76209
ing6_Resazurin["Resazurin"]
CultureMech_000690 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000690 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000690 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_KCl["KCl"]
CultureMech_000690 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_NaBr["NaBr"]
CultureMech_000690 --> ing10_NaBr
CHEBI_63004["CHEBI:63004"]
ing10_NaBr -.-> CHEBI_63004
ing11_H3BO3["H3BO3"]
CultureMech_000690 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000690 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_000690 --> ing13_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing13_SrCl2_x_6_H2O -.-> CHEBI_36385
ing14_KI["KI"]
CultureMech_000690 --> ing14_KI
CHEBI_8346["CHEBI:8346"]
ing14_KI -.-> CHEBI_8346
ing15_Double_distilled_water["Double distilled water"]
CultureMech_000690 --> ing15_Double_distilled_water
CHEBI_15377["CHEBI:15377"]
ing15_Double_distilled_water -.-> CHEBI_15377
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
107.7 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 80.0, 27.7] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 | Yeast extract | 0.2 G_PER_L | ||
| 4 | Peptone | 0.2 G_PER_L | ||
| 5 | Starch | 5 G_PER_L |
CHEBI:28017
starch
|
|
| 6 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
5e-07 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.6 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 11 |
NaBr
Br.Na 102.894 g/mol
|
0.1 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 14 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.015 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 15 |
KI
I.K 166.002 g/mol
|
5e-05 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 16 |
Double distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Preparation
- Prepare the medium without the Na2S x 9 H2O by boiling and cooling under a nitrogen atmosphere. Adjust the pH to 6.5 - 7.0. Dispense into Hungate tubes or serum bottles fitted with butyl rubber stoppers. Autoclave and reduce the medium before use by adding the appropriate amount of Na2S x 9 H2O from a neutralised, sterile stock solution.
- Adjust pH to 5.5 - 6.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.787485Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1231 |
| 2026-03-13T03:09:23.571011Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:57.480762Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000690 |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.765170+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOPLASMA CONTRACTILE MEDIUM" to "haloplasma_contractile_medium" for consistent matching |
| 2026-05-20T06:01:22.264948+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.5-7' -> {'min': 6.5, 'max': 7.0} |
| 2026-06-05T07:41:08.081349+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.621988+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |